牛流行热在天祝白牦牛中流行病学的调查

发布时间：2018-02-01 06:32

本文关键词： 牛流行热病毒白牦牛流行病学调查　出处：《中国兽医科学》2017年09期 　论文类型：期刊论文

【摘要】：为了解牛流行热在天祝白牦牛中的流行状况,本研究从甘肃省武威市天祝藏族自治县的7个乡镇分别随机抽样采集了224头白牦牛的抗凝血和血清,应用间接ELISA抗体检测试剂盒检测血清中牛流行热病毒特异抗体,应用实时荧光定量RT-PCR、RT-PCR方法检测牛流行热病毒G基因并进行序列验证。结果表明,7个乡镇的白牦牛均存在牛流行热病毒感染,抗体阳性率为45.09%;荧光定量RT-PCR的检出阳性率为37.5%;以RT-PCR扩增G基因3’端420 bp区段测序并构建系统发育树表明,白牦牛群流行的BEFV毒株与中国大陆1976年分离的JB76H毒株亲缘关系较近,为BEFV基因Ⅰ型。本研究首次证实白牦牛在自然状态下可感染牛流行热病毒,可能是重要的牛流行热病毒宿主。
[Abstract]:In order to understand the epidemic status of cattle epidemic fever in Tianzhu white yak, the anticoagulant and serum samples of 224 white yaks were collected randomly from 7 villages and towns in Tianzhu Tibetan Autonomous County, Wuwei City, Gansu Province. Indirect ELISA antibody detection kit was used to detect bovine epidemic fever virus specific antibody in serum and real-time fluorescence quantitative RT-PCR was used. RT-PCR method was used to detect the G gene of bovine epidemic fever virus. The results showed that all the white yaks in 7 towns were infected with bovine epidemic fever virus, and the positive rate of antibody was 45.09; The positive rate of fluorescence quantitative RT-PCR was 37.5%. The 3'end 420bp fragment of G gene was amplified by RT-PCR and the phylogenetic tree was constructed. The BEFV strains in white yaks were closely related to the JB76H strains isolated in mainland China in 1976. This study first confirmed that white yak can infect bovine epidemic fever virus in natural state, which may be an important host of bovine epidemic fever virus.
【作者单位】：中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室;江苏省动物重要疫病与人兽共患病防控协同创新中心;
【基金】：国家农业公益性行业科研专项——重要牛羊虫媒病毒病防控关键技术研究与应用(201303035) 国家肉牛牦牛产业技术体系(NBCIS)(CARS-38) 农业科技创新工程(ASTIP)(2014ZL010)
【分类号】：S858.23
【正文快照】： 牛流行热(bovine ephemeral fever,BEF)是由牛流行热病毒(bovine ephemeral fever virus,BEFV)引起的黄牛、奶牛及水牛的一种急性非接触性传染病,通过媒介昆虫传播,可导致奶牛产奶量降低、影响牛肉品质及牛的使役能力,从而造成重大的经济损失。该病的流行范围广泛,包括埃及、

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