1株HA蛋白S145变异H9N2亚型禽流感病毒的分离鉴定与HA基因序列分析

发布时间：2018-02-02 08:30

本文关键词： 禽流感病毒 HN亚型 HA基因 S位点遗传进化　出处：《中国兽医学报》2017年04期 　论文类型：期刊论文

【摘要】：从山东某发病养殖场采集疑似病料,经RT-PCR检测为H9N2亚型禽流感阳性,采用鸡胚尿囊腔接种法分离病毒(SD089),并进行HA、HI试验和HA基因扩增、测序以及序列的同源性和遗传进化分析。结果显示,分离的SD089株具有血凝特性但不能被H9亚型禽流感标准阳性血清所抑制,其HA基因推导的氨基酸序列与参考毒株相比,第145位由S漂变为N,导致增加糖基化位点NGT,表明S145是血凝抑制抗体结合位点。SD089株属于BJ94分支,与2010 2014年大部分山东流行毒株的核苷酸和氨基酸序列相似性分别为88.9%~97.5%和89.9%~99.6%,而与疫苗株SD696分别降至88.0%和89.1%。试验表明,分离的SD089株HA基因在分子水平上发生了变异,S145位点的突变提示低致病性禽流感的防制面临新的挑战。
[Abstract]:The suspected samples were collected from a disease farm in Shandong Province. The H9N2 subtype avian influenza was detected by RT-PCR. The virus SD089 was isolated from chicken embryo by allantoic cavity inoculation, and HA was carried out. HI test and HA gene amplification, sequencing and sequence homology and genetic evolution analysis. The isolated SD089 strain had hemagglutination property but could not be inhibited by H9 subtype avian influenza standard positive serum. Compared with the reference strain, the deduced amino acid sequence of HA gene of the isolate changed from S bleaching to N. The results showed that S145 was the binding site of hemagglutination inhibition antibody. SD089 belonged to the BJ94 branch. In 2014, the nucleotide sequence and amino acid sequence similarity of most of the strains were 88.997. 5% and 89. 9% respectively. The results showed that the HA gene of the isolated SD089 strain was mutated at molecular level. The S 145 mutation suggests a new challenge to the prevention and control of low-pathogenic avian influenza.
【作者单位】：广州市华南农大生物药品有限公司;
【分类号】：S852.65
【正文快照】： 禽流感病毒(avian influenza virus,AIV)是正黏病毒科流感病毒属A型流感病毒,是危害养禽业和人类健康的重要病原,目前已发现有16个血凝素(HA)亚型和10个神经氨酸酶(NA)亚型[1]。H9N2亚型病毒为低致病性AIV,首次于1966年从患温和呼吸道病的火鸡中分离到,之后在世界范围内广泛流

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