体外培养条件下不同长链脂肪酸对山羊瘤胃原虫群体结构和原虫吞噬细菌循环的影响

发布时间：2018-02-02 20:11

本文关键词： 脂肪酸不饱和键原虫吞噬细菌　出处：《动物营养学报》2017年08期 　论文类型：期刊论文

【摘要】：本试验旨在研究体外培养条件下不同长链脂肪酸对山羊瘤胃原虫群体结构和原虫吞噬细菌循环的影响。试验选取6种不同不饱和度的长链脂肪酸[分别为硬脂酸(A组)、油酸(B组)、亚油酸(C组)、α-亚麻油酸(D组)、花生四烯酸(E组)、二十碳五烯酸(F组),不饱和键个数从0~5依次递增],各脂肪酸的添加水平为底物的3%,进行体外培养。在体外培养10 h后测定原虫吞噬细菌速率,在体外培养24 h后测定细菌、原虫密度以及细菌蛋白质、原虫蛋白质含量。结果表明:除F组头毛虫属(Ophryoscolecinae)比例显著高于B和D组(P0.05)外,不同长链脂肪酸的添加对原虫中厚毛虫属(Dasytricharuminantium)、内纤毛虫属(Entodinium)、双毛虫属(Diplodiniinae)、等毛虫属(Isotrichidae)、前毛虫属(Epidinium)比例的影响均不显著(P0.05)。原虫密度以A组最高,并显著高于B组(P0.05);细菌密度以D组最高,并显著高于A、B、E、F组(P0.05)。原虫吞噬细菌速率A~F组依次为244.50、236.51、229.60、189.04、200.51、174.24 cells/(cell·h);另外,以D组的细菌周转率(0.68%)最低,且其细菌周转时间(146.92 h)最长。所估算的细菌蛋白质循环量以D[126.75 mg/(d·头)]和F组[131.63 mg/(d·头)]相对较低。因此,在本试验基础上认为,体外培养条件下α-亚麻油酸抑制山羊瘤胃原虫吞噬细菌效果较好。
[Abstract]:The purpose of this study was to study the effects of different long chain fatty acids on the population structure of rumen protozoa and the phagocytic cycle of protozoa in vitro. Six kinds of long chain fatty acids with different unsaturation were selected. [They were stearic acid group A, oleic acid group B, linoleic acid group C, 伪 -linolenic acid group D, arachidonic acid group E, group 20 carbapentaenoic acid F). The number of unsaturated bonds increased from 0 to 5 in turn. The fatty acid levels were 3% of the substrate and cultured in vitro. The phagocytic rate of protozoa was measured after 10 hours of culture in vitro. Bacteria, protozoa density and bacterial protein were measured after 24 h culture in vitro. The results showed that the percentage of Ophryoscolecinae in group F was significantly higher than that in groups B and D (P 0.05). Different long chain fatty acids were added to protozoa, Dasytricharuminantium and Entodinium. Diplodiniinaeae, Isotrichida). The effect of Epidinium ratio was not significant. The density of protozoa was the highest in group A and significantly higher than that in group B. The bacterial density in group D was the highest and significantly higher than that in group A (P 0.05). The phagocytic rate of protozoa in group A was 244.50 (236.51) and 229.60, respectively. 189.04 (200.51A) 174.24 cells/(cell 路hmb; In addition, group D had the lowest bacterial turnover rate (0.68%) and the longest bacterial turnover time (146.92 h). [126.75 mg/(d 路head) and group F. [The results showed that 伪 -linolenic acid could inhibit the phagocytosis of goat rumen protozoa in vitro.
【作者单位】：扬州大学动物科学与技术学院;
【基金】：国家自然基金项目(31672446) 江苏省自然科学基金基础研究项目(BK20151312) 江苏省优势学科
【分类号】：S827.5
【正文快照】： 瘤胃微生物蛋白质(MCP)是反刍动物的主要氮素来源之一,能提供蛋白质需要量的40%~80%,其合成量的高低可以反映瘤胃中微生物的数量和微生物蛋白质合成的效率[1],并且与瘤胃对氮源的有效利用有关[2]。瘤胃原虫对细菌的吞噬和消化所导致的瘤胃内氮循环无疑降低了氮的利用效率[3-5]

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