不同因素对绵羊体外胚胎生产的影响

发布时间：2018-02-03 06:35

本文关键词： 绵羊体外胚胎生产褪黑素胚胎凋亡　出处：《内蒙古农业大学》2016年硕士论文　论文类型：学位论文

【摘要】：本论文主要研究了不同因素对绵羊体外胚胎生产的影响,主要分为以下几个方面：1.在绵羊卵母细胞成熟液中添加不同浓度的褪黑素(0、10-5、10-7、10-9mol/L),体外培养24h后,观察各试验组的成熟率。结果表明,褪黑素浓度为10-7M组成熟率最高,但与其他组相比无显著性差异(P0.05)。各组卵母细胞成熟后进行孤雌激活,体外培养,结果显示,四个组之间卵裂率和囊胚率均无显著差异(P0.05),但三个试验组的囊胚凋亡指数(12.22～18.18%)显著低于无添加的对照组(21.30%,P0.05)。其中,10-9mol·L-1浓度组的凋亡指数最低。2.比较精液常温保存和低温保存对体外受精的影响。采集新鲜的公羊精液,经过稀释后分别进行常温保存和低温保存,然后体外受精,结果表明,低温保存组卵裂率和囊胚率均略高于常温保存组,但两组无显著性差异(P0.05)。3.比较受精液中不同血清浓度对体外受精的影响。分别用含有2%和20%发情母羊血清的受精液进行体外受精,结果表明,两试验组卵裂率和囊胚率均无显著性差异(P0.05)。4.比较两种不同的精液稀释保存液对体外受精效果的影响。结果表明,利用稀释保存液LTGC和SOF常温保存处理后的精子,进行体外受精后,两试验组卵裂率和囊胚率均无显著性差异(P0.05)。5.卵母细胞体外成熟并孤雌激活后,移入添加了不同浓度褪黑素(0、10-5、10-7、10-9mol·L-1)的培养液中进行体外培养,结果表明,四个组之间的卵裂率并无显著差异(P0.05),10-7、10-9mol·L-1两个试验组的囊胚率要显著高于其他两组,但两个组间无差异。10-5、10-7、10-9mol·L-1浓度组的胚胎凋亡指数显著低于对照组(P0.05),其中10-5mol·L-1组的(17.56%)显著高于10-7、10-9浓度组(13.64%、11.62%,P0.05)。
[Abstract]:In this paper, we studied the effect of different factors on sheep embryo production in vitro, mainly divided into the following aspects: 1. Adding different concentrations of melatonin 10 ~ (-5) into ovine oocyte maturation fluid. After 24 hours of culture in vitro, the maturation rate of each experimental group was observed. The results showed that melatonin concentration was the highest in 10-7 M group. However, there was no significant difference compared with other groups (P 0.05). After maturation of oocytes in each group, parthenogenetic activation was carried out and cultured in vitro, the results showed that. There was no significant difference in cleavage rate and blastocyst rate among the four groups (P 0.05). But the apoptotic index of blastocyst in the three experimental groups was significantly lower than that in the control group without addition. The apoptosis index was the lowest in 10-9 mol 路L-1 group. The effects of cryopreservation and room temperature preservation on in vitro fertilization were compared. Fresh ram semen was collected. The cleavage rate and blastocyst rate of cryopreservation group were slightly higher than that of normal temperature preservation group. However, there was no significant difference between the two groups (P 0.05). The effect of different serum concentrations in semen on in vitro fertilization was compared. Semen containing 2% and 20% estrous estrus serum were used for in vitro fertilization. The results showed that there was no significant difference in cleavage rate and blastocyst rate between the two groups. The spermatozoa treated by dilution preservation solution LTGC and SOF were stored at room temperature for in vitro fertilization. There was no significant difference in cleavage rate and blastocyst rate between the two groups. After maturation and parthenogenetic activation of oocytes in vitro, different concentrations of melatonin 10 ~ (-5) + 10 ~ (-7) were added. The results showed that there was no significant difference in cleavage rate among the four groups. The blastocyst rate of 10-9 mol 路L -1 group was significantly higher than that of the other two groups, but there was no difference between the two groups. The embryonic apoptosis index of the 10-9 mol 路L-1 group was significantly lower than that of the control group (P 0.05), and that of the 10-5 mol 路L -1 group was significantly higher than that of the 10-5 mol 路L -1 group. In the 10-9 concentration group, 13.64% and 11.62%, P 0.05%.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S826

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