缺氧影响奶牛子宫内膜上皮细胞OPN和cyclinG1的表达

发布时间：2018-02-03 08:35

本文关键词： 奶牛子宫内膜上皮细胞缺氧 OPN cyclinG1　出处：《华中农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：我国奶牛养殖业在国民经济中的地位十分重要,但奶牛不孕严重制约我国奶牛繁殖的数量和生产性能。胚胎的形成对妊娠的成败起关键作用,早期胚胎着床困难及流失是奶牛不孕的主要原因。早期胚胎的正确着床需要有同步发育的子宫来接受。OPN与母胎免疫和蜕膜化等有关。而cyclin G1跟细胞的增值和分化有关。研究显示胎盘形成初期子宫是处于缺氧状况的。因此在早期妊娠缺氧可能对胎盘形成起着非比寻常的作用。本实验的主要目的是观察缺氧条件下OPN和cyclin G1的表达情况。本试验用Co Cl2制造细胞缺氧,利用Co Cl2的浓度不同来控制细胞缺氧,然后把体外培养的奶牛子宫内膜上皮细胞置于不同的浓度的COCl2下作用不同的时间。然后测定不同情况下的OPN和cyclin G1在子宫内膜上皮细胞的表达情况。揭示其表达规律,阐明其中现象,从而在生产实践中直到提高奶牛胚胎成化率,提高我国奶业产量。本实验是采用浓度-时间双重条件,Co Cl2浓度设定100、200和400μmol/L,时间设定3、6和12h。两两组合,总共9组。通过RT-PCR测定细胞中OPN和cyclin G1的表达情况。实验结果显示在不同浓度和时间下(100μmol/L,200μmol/L,400μmol/L;3h,6h,12h),OPN m RNA的表达量具有明显规律,在同一Co Cl2浓度下,随着时间的增加表达量增加,而且高浓度Co Cl2下表达量低于同一时间下低浓度的表达量;而cyclin G1在100μmol/L情况在随着时间延长,表达量增加,而在200和400μmol/L的情况下随着时间的延长而表达量下降。结论:缺氧均能够调节OPN和cyclin G1 m RNA的在子宫内膜上皮细胞中的表达。OPN的表达随着氧浓度越低和作用时间的延长而显著升高,而cyclin G1随着缺氧程度加重和缺氧时间的延长表达量均显著下降。
[Abstract]:The status of dairy cattle breeding industry in China is very important in the national economy, but the infertility of dairy cows seriously restricts the number and production performance of dairy cows in China. The formation of embryos plays a key role in the success or failure of pregnancy. The difficulty of implantation and loss of early embryos are the main causes of infertility in dairy cows. The correct implantation of early embryos requires synchronous uterus to receive. OPN is related to maternal and fetal immunity and decidualization. G1 is associated with cell proliferation and differentiation. Studies have shown that the uterus is anoxic in the early stages of placental formation. Therefore, hypoxia in early pregnancy may play an unusual role in placental formation. The main purpose of this study is to. Observation of OPN and cyclin under hypoxia. In this study, we used Co Cl2 to create hypoxia in cells. Different concentrations of Co Cl2 were used to control cell hypoxia. Then the cultured bovine endometrial epithelial cells were exposed to different concentrations of COCl2 for different time. OPN and cyclin were measured under different conditions. Expression of G1 in endometrial epithelial cells. In order to improve the rate of embryo formation and increase the yield of dairy industry in our country, the experiment was carried out under the condition of concentration and time, so that the concentration of Co Cl2 was set up in the production practice. 200 渭 mol / L and 400 渭 mol / L, time set at 3, 6 and 12 h. The expression of OPN and cyclin G1 was measured by RT-PCR. The results showed that the expression of OPN and cyclin G1 was 100 渭 mol/L at different concentration and time. 200 渭 mol / L, 400 渭 mol / L; The expression of OPNm RNA at the same concentration of Co Cl2 increased with the increase of time. The expression of Co Cl2 at high concentration was lower than that at low concentration at the same time. However, the expression of cyclin G1 at 100 渭 mol/L increased with time. At 200 and 400 渭 mol/L, the expression decreased with time. Conclusion: hypoxia can regulate both OPN and cyclin G1 m. The expression of RNA in endometrial epithelial cells increased significantly with the decrease of oxygen concentration and the prolongation of action time. However, the expression of cyclin G1 decreased significantly with the increase of hypoxia and the prolongation of hypoxia time.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.23

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