滁州活禽市场鸡源沙门菌的分离鉴定与药敏试验

发布时间：2018-02-03 22:18

本文关键词： 沙门菌鸡分离鉴定药敏试验　出处：《动物医学进展》2017年06期 　论文类型：期刊论文

【摘要】：从安徽滁州地区某活禽市场采集鸡的肛拭子样品110份,接种到麦康凯培养基分离纯化,再选取典型菌落进行三糖铁、吲哚、甲基红、VP等生化试验及16SrRNA的PCR鉴定,最后对沙门菌分离株进行药敏试验。110份样品经分离纯化得到29株疑似沙门菌菌株,通过生化试验及16SrRNA的分子鉴定,共分离到23株沙门菌,检出率为20.9%;药敏试验表明,沙门菌分离株对链霉素和阿米卡星的耐药率相对较低,而对庆大霉素、大观霉素、哌拉西林的耐药率均高达82.61%。对克林霉素的耐药率为100%。
[Abstract]:110 chicken anal swab samples were collected from a live poultry market in Chuzhou, Anhui Province, and inoculated into wheat Kang Kai medium for isolation and purification, and then selected typical colonies for triose iron, indole and methyl red. VP and other biochemical tests and 16s rRNA PCR identification. Finally, the drug sensitivity test of Salmonella isolates. 110 samples were isolated and purified, 29 suspected Salmonella strains were obtained. By biochemical test and molecular identification of 16s rRNA, a total of 23 Salmonella strains were isolated, the detection rate was 20.9; Drug sensitivity test showed that the resistance rate of Salmonella isolates to streptomycin and amikacin was relatively low, but to gentamicin and spectinomycin. The resistance rates of piperacillin and clindamycin were as high as 82.61 and 100 respectively.
【作者单位】：安徽科技学院动物科学学院;河南省驻马店市畜牧局;
【基金】：安徽省教育厅自然科学研究重点项目(KJ2016A822) 安徽科技学院自然科学研究项目(ZRC2016497) 安徽省科技攻关计划项目(1301zc03088)
【分类号】：S852.61
【正文快照】： 沙门菌是肠杆菌科的一种革兰阴性菌,广泛存在于人和多种动物肠道中,例如牛、马、猪等哺乳动物,鸡、鸭、鹅等家禽,以及其他爬行动物和两栖动物[1]。大多数沙门菌对人和动物具有致病性,其血清型有2500多个,并且不同血清型对各种动物的致病性也不相同,能够引起常见多发的人畜共患

【相似文献】