饲料添加剂Lucta对泌乳早期奶牛生产性能和健康的影响
本文关键词: 能量负平衡 饲料添加剂 生产性能 抗氧化能力 免疫能力 出处:《南京农业大学》2015年硕士论文 论文类型:学位论文
【摘要】:本研究包括三个系列:1、Lucta对泌乳早期奶牛生产性能和健康的影响;2、Lucta在泌乳早期奶牛的生产性应用;3、Lucta对泌乳早期奶牛影响的机理初探。1、Lucta对泌乳早期奶牛生产性能和健康的影响选取泌乳早期奶牛98头分别进行两个实验:其中,实验一(68头)为分娩一---二个月奶牛,实验二(30头)为分娩一个月奶牛。在两个实验中奶牛均被分为两组:Lucta组(每天分三次共饲喂Lucta 80g)和Control组,两组奶牛均自由采食饲料和饮水,饲喂实验持续120天。实验期定期测定其采食量,产奶量,乳品质,发情与配种以及体况评分等相关指标。统计结果表明,Lucta可显著增加奶牛的采食量;Lucta可显著增加分娩1个月奶牛以及头胎奶牛的产奶量;Lucta可显著提高奶牛的体况评分,Lucta可降低奶牛受孕所需的配种次数和分娩至受孕的时间间隔,但差异不显著;Lucta可降低奶牛隐性乳房炎的发病率,但差异不显著。2、Lucta在泌乳早期奶牛的生产性应用选取泌乳早期奶牛220头用于生产性应用研究,奶牛被分为两组:Lucta组(每天分三次共饲喂Lucta 80 g)和Control组,两组奶牛均自由采食饲料和饮水,饲喂持续120天。实验期定期测定其采食量,产奶量,乳品质以及体况评分等相关生产指标。统计结果表明,Lucta可增加奶牛的采食量;Lucta可增加奶牛的产奶量;Lucta可显著提高奶牛的体况评分。3、Lucta对泌乳早期奶牛生产性能影响的机理初探对系列一中98头泌乳早期奶牛,在实验期第30天,晨饲前尾静脉采血,分离血清,于-20冰箱冻存。采用酶免法,放免法和比色法等方法对血清中能量代谢指标,抗氧化指标,繁殖相关指标以及免疫相关指标进行测定。测定结果:能量代谢指标结果显示Lucta可增加血清中T3、T4、Insulin、Glu和PUN含量,降低BHBA、NEFA、TG和T-CHO含量;抗氧化指标结果显示Lucta可增加血清中SOD和T-AOC含量,降低MDA含量;繁殖相关指标结果显示Lucta可增加血清中LH、FSH和E2;免疫相关指标显示Lucta可增加血清中IL-2含量,降低IL-6含量。上述结果表明,Lucta饲喂泌乳早期奶牛可提高其能量代谢水平、抗氧化水平、繁殖性能和免疫能力。
[Abstract]:This study included the effects of three series 1: 1 Lucta on the performance and health of early lactation cows. (2) the productive application of Lucta in early lactation; Study on the effect of Lucta on the performance and Health of early lactating Dairy cows two experiments were carried out on 98 early lactating cows. Experiment 1: 68 cows were given birth for one to two months. In experiment 2, 30 cows were divided into two groups: two groups: Lucta 80g (three times a day) and Control group. The feeding experiment lasted for 120 days. The feed intake, milk yield, milk quality, estrus and breeding, and body condition score were measured regularly in both groups. Lucta significantly increased the feed intake of dairy cows. Lucta could significantly increase the milk production of dairy cows and first-born cows after 1 month of delivery. Lucta could significantly improve the body condition score of dairy cows and reduce the frequency of mating and the interval between delivery and conception, but the difference was not significant. Lucta can reduce the incidence of recessive mastitis in dairy cows, but the difference is not significant. The cows were divided into two groups: Lucta 80g and Control groups. The cows in the two groups were fed free feed and drinking water. The feeding lasted for 120 days. The feeding intake, milk yield, milk quality and body condition score were measured periodically during the experiment period. The statistical results showed that Lucta could increase the feed intake of dairy cows. Lucta can increase milk yield of dairy cows. The effect of Lucta on the performance of early lactating Dairy Dairy cattle; on the 30th day of the experimental period, 98 early lactating dairy cows in the first middle school of China were treated with Lucta. The serum was collected from tail vein before morning feeding and frozen in -20 refrigerator. The indexes of energy metabolism and antioxidation in serum were determined by enzyme immunoassay, radioimmunoassay and colorimetry. The results showed that Lucta could increase the content of Glu and PUN in serum. The contents of TG and T-CHO were decreased. The results of antioxidant index showed that Lucta could increase the contents of SOD and T-AOC in serum and decrease the content of MDA. The results of reproductive related indexes showed that Lucta could increase serum LHH FSH and E2; The immune-related indexes showed that Lucta could increase the content of IL-2 in serum and decrease the content of IL-6. Antioxidant levels, reproductive performance and immune capacity.
【学位授予单位】:南京农业大学
【学位级别】:硕士
【学位授予年份】:2015
【分类号】:S823
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