禽呼肠孤病毒感染后SPF鸡关节中Toll样受体的mRNA转录水平变化

发布时间：2018-02-05 01:09

本文关键词： 禽呼肠孤病毒鸡Toll样受体关节荧光定量PCR方法　出处：《中国兽医学报》2017年03期 　论文类型：期刊论文

【摘要】：禽呼肠孤病毒(avian reovirus,ARV)感染主要引起鸡的病毒性关节炎和免疫抑制等,造成养禽业巨大经济损失。近年来Toll样受体(Toll-like receptors,TLRs)在病原体感染中的作用受到广泛关注。在本试验中,将ARV S1133毒株经足垫注射,接种7日龄SPF鸡,感染后1、3、5、7、14、21、28和35d采集鸡关节样品,利用荧光定量PCR方法检测TLR3、TLR5、TLR7、TLR15和TLR21在关节组织中的mRNA表达水平变化,探讨ARV感染对鸡关节中上述TLRs mRNA表达量的影响。结果表明,ARV可引起,尤其感染早期鸡只出现足垫肿胀的症状,并且关节中这些TLRs在感染后3d显著上调并达到峰值(P0.05或P0.01),随后表达量开始下调,在感染后14d表达量显著下调到最低值(P0.05或P0.01),然后直至试验结束,恢复至与对照组基本持平。以上结果提示TLR3、TLR5、TLR7、TLR15和TLR21可能参与了ARV感染对鸡关节的炎症过程,本试验结果为进一步阐明禽呼肠孤病毒的致病机理提供了依据。
[Abstract]:Avian reovirus (ARV) infection mainly causes viral arthritis and immunosuppression in chickens. In recent years, Toll like receptor Toll-like receptors. In this experiment, ARV S1133 strain was injected with foot pad and inoculated into 7 day old SPF chicken. Chicken joint samples were collected on the 28th and 35th day, and TLR3 TLR5 and TLR7 were detected by fluorescence quantitative PCR. The changes of mRNA expression of TLR15 and TLR21 in joint tissue were studied to investigate the effect of ARV infection on the expression of TLRs mRNA in chicken joints. ARV may cause, especially in the early stage of infection chicken foot pad swelling symptoms, and these TLRs in the joints at 3 days after infection significantly up-regulated and reached the peak value of P0.05 or P0.01). Then the expression began to down-regulate, at the 14th day after infection, the expression decreased significantly to the lowest value (P0.05 or P0.01A), and then to the end of the experiment. The above results suggested that TLR3, TLR5, TLR7, TLR15 and TLR21 might be involved in the inflammation of chicken joints caused by ARV infection. The results provide a basis for further elucidating the pathogenic mechanism of avian reovirus.
【作者单位】：广西壮族自治区兽医研究所广西兽医生物技术重点实验室;广西大学动物科学技术学院;
【基金】：国家自然科学基金资助项目(31660715) 广西自然科学基金资助项目(2014GXNSFCA118006) 广西水产畜牧兽医局科技项目(桂渔牧科201452003) 国家“万人计划”领军人材专项资助项目(2016-37)
【分类号】：S858.31
【正文快照】： *Corresponding author,E-mail:xiezhixun@126.com禽呼肠孤病毒(avian virus,ARV)属于呼肠孤病毒科正呼肠孤病毒属双股分节段RNA病毒,1954年首次从鸡体中分离被发现[1]。该病毒感染后在临床上主要表现为腱鞘炎型、败血型、吸收不良综合征型等,也可引起鸡组织器官出现广泛性炎

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