鸡脾转移因子对鸡肠道抗氧化能力的影响

发布时间：2018-02-05 16:47

本文关键词： 转移因子鸡肠道抗氧化　出处：《畜牧兽医学报》2017年10期 　论文类型：期刊论文

【摘要】：旨在通过分析鸡脾转移因子(TF)对鸡肠道抗氧化能力的影响,探讨其调控鸡生长、免疫的作用机制。选用1日龄SPF鸡100羽,自5日龄开始在饮水中加入鸡脾转移因子,饲喂1周,停喂1周,饲喂分为4个剂量组,分别为0.05(最低剂量组)、0.10(低剂量组)、0.25(中剂量组)、1mL·只-1(高剂量组)剂量组及正常对照组,饲养至12日龄(连续用药1周)及19日龄(停喂1周)时各取50羽,分别称量其体重,并测量十二指肠、空肠、回肠和直肠的抗氧化能力。结果表明,口服鸡脾转移因子1周,就显示出升高体重的趋势,但是效果尚不明显。高剂量组可显著增加12日龄雏鸡十二指肠、空肠、回肠、直肠SOD(17.07%~31.54%)、CAT(16.98%~25.77%)、GSH-PX(13.99%~23.59%)的活力,以及总抗氧化能力T-AOC(16.48%~43.16%),降低各段MDA含量(P0.05);中剂量组显著增加各肠段的T-AOC和SOD活性、CAT活力(回肠除外)、GSH-PX活力(十二指肠除外);且低、中剂量组均显著降低各肠段的MDA含量(P0.05)。停喂一周后,尽管各剂量组对各肠段T-AOC、CAT、GSH-PX、SOD、MDA活性的改变较12日龄有不同程度的降低,但是某些肠段较对照组仍然作用显著(P0.05)。结果提示,鸡脾转移因子作为新型饲料添加剂能显著提高鸡肠道抗氧化应激能力。
[Abstract]:The aim of this study was to analyze the effect of spleen transfer factor (TFF) on the antioxidant capacity of chicken intestine, and to explore the mechanism of its regulation of chicken growth and immunity. One day old SPF chickens were selected. From 5 days of age, chicken spleen transfer factor was added to drinking water, fed for 1 week, stopped feeding for 1 week, and was divided into 4 dosage groups, 0.05 (lowest dose group 0.10 (low dose group)). 0.25 (medium dose group) 1 mL 路mouse -1 (high dose group) and normal control group were fed to 12 days of age (continuous treatment for 1 week) and 19 days of age (stop feeding for 1 week). The antioxidant capacity of duodenum jejunum ileum and rectum were measured respectively. But the effect was not obvious. The high dose group could significantly increase the duodenum, jejunum, ileum, rectum SOD 17.07 and 31.54 in 12-day-old chicks. The activity of GSH-PX 13.99 is 23.59. The total antioxidant capacity (T-AOC) was 16.48%, and the content of MDA in each segment was decreased (P 0.05). In the middle dose group, T-AOC and SOD activities were significantly increased (except the ileum, GSH-PX and duodenum, respectively). In the low and middle dose groups, the MDA content of each intestinal segment was significantly decreased (P 0.05). One week after stopping feeding, the GSH-PXX SOD of T-AOC Con CATX was significantly decreased in each dose group. The change of MDA activity was lower than that of 12 days old, but some intestinal segments still had a significant effect on P0.05A compared with the control group. The results suggested that there was no significant difference between the two groups. As a new type of feed additive, chicken spleen transfer factor can significantly improve the ability of antioxidant stress in chicken intestine.
【作者单位】：中国农业大学动物医学院;山东省农业科学院家禽研究所;中国牧工商集团总公司;
【基金】：国家自然科学基金(31372332;31272483)
【分类号】：S831

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