口蹄疫O型、A型、Asia I型三价灭活疫苗猪、牛、羊抗体消长规律分析

发布时间：2018-02-06 03:06

本文关键词： 抗体消长液相阻断ELISA 免疫非结构蛋白3ABC　出处：《甘肃农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：口蹄疫(Foot-and-Mouth Disease)是引起偶蹄动物的热性、急性、高度接触性的传染病,如果一旦发病其发病率可以到达100%,死亡率达到20%~50%,严重影响了全球畜牧业的健康发展。发达国家防控口蹄疫主要是采用全面捕杀感染动物的方式,我国对口蹄疫的防控措施主要采用的是疫苗免疫的方式。牛是FMDV最易感动物,尤其是奶牛,具有排毒量大的特点。羊患此病后临床症状较其他偶蹄动物相比并不明显,故成为不易被发现的传染源存在,而猪对FMDV非常敏感,患病猪可以产生大量气溶性病毒,一头病猪其气源性排毒量相当于1000~3000头患病牛的释放量,故检测猪、牛、羊抗体消长变化规律及感染情况对FMD的防控具有极大的指示作用。3ABC是FMDV编码的非结构蛋白,3ABC抗体被用来作为鉴别诊断以区分感染和非感染动物。本研究选取不同日龄猪群作为实验对象,严格按照免疫程序进行免疫,在检测抗体效价变化的同时重点研究口蹄疫疫苗免疫次数与3ABC阳性率关系,结果显示在猪体内免疫次数与3ABC阳性率之间并无明显线性关系。针对牛群分别于第0天、第157天进行进行两次免疫,免疫后分4个时间点采血并检测O、A和Asia1型抗体的变化趋势,结果表明使用实验组疫苗在首免及首免后157天免疫即可产生持续大于1:64滴度的抗体水平,能有效预防牛群口蹄疫的发生。针对羊群分别于第0天、第177天进行进行两次免疫,免疫后分6个时间点采血并检测三型抗体的变化趋势,结果表面使用实验组疫苗在首免及首免后177免疫两次即可产生大于持续1:64滴度的抗体水平,对羊群起到较好的保护作用。本研究结合生产实践,对规模化猪场、牛场、羊场口蹄疫三价灭活疫苗抗体消长规律做了较为深入的研究,具有一定理论指导意义和实际应用价值。
[Abstract]:Foot-and-mouth disease (Foot-and-Mouth disease) is a feverish, acute and highly contact infectious disease in cloven-hoofed animals. Once infected, it can reach 100%. The mortality rate has reached 20%, which has seriously affected the healthy development of animal husbandry in the world. The prevention and control of foot-and-mouth disease in developed countries mainly adopts the way of killing and killing infected animals. Vaccination is the main method of prevention and control of foot-and-mouth disease in China. Cattle are the most susceptible animals of FMDV, especially dairy cows. The clinical symptoms of sheep with this disease are not obvious compared with other cloven-hoofed animals, so it is difficult to find the source of infection, and pigs are very sensitive to FMDV. Infected pigs can produce a large number of air-soluble virus, a sick pig whose airborne excretion amount is equivalent to 1000 or 3000 sick cattle release, so the detection of pigs, cattle. The change rule of sheep antibody and infection condition have a great indicative effect on the prevention and control of FMD. 3ABC is a non-structural protein encoded by FMDV. 3ABC antibody was used as differential diagnosis to distinguish infected and non-infected animals. In this study, pigs of different ages were selected as experimental subjects and immunized strictly according to the immune procedure. The relationship between the number of times of immunization and the 3ABC positive rate of foot-and-mouth disease vaccine was studied at the same time as the change of antibody titer. The results showed that there was no significant linear relationship between the number of immunizations in pigs and the positive rate of 3ABC. The cows were immunized twice on day 0 and day 157 respectively. The blood samples were collected at four time points after immunization and the changes of OMA and Asia1 antibodies were detected. The results showed that the antibody level of above 1: 64 titers could be produced after the first immunization and 157 days after the first immunization, which could effectively prevent the occurrence of foot-and-mouth disease in cattle. Two immunizations were carried out on the 177th day. The blood samples were collected at 6 time points after immunization and the trend of type III antibody was detected. Results the antibody level was higher than 1: 64 titers after the first immunization and 177 immunization after the first immunization. The vaccine had a better protective effect on sheep. This study combined with production practice. The growth and decline rule of inactivated vaccine antibody against foot-and-mouth disease (FMD) in large scale pig farm, cattle farm and sheep farm were studied in depth, which has a certain theoretical significance and practical application value.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S852.4

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