FABP3及FABP4基因SNP检测及其与秦川肉牛肉质性状的关联性分析

发布时间：2018-02-09 00:43

本文关键词： 秦川肉牛 FABP3基因 FABP4基因多态性关联分析　出处：《西北农林科技大学》2017年硕士论文　论文类型：学位论文

【摘要】：脂肪酸结合蛋白3(fat acid binding proteins 3,FABP3)和脂肪酸结合蛋白4(fat acid binding proteins 4,FABP4)是FABPs家族的重要成员。FABPs的主要作用是调节脂肪酸摄取和细胞内转运,尽管蛋白质序列有很大的差异,但基因结构相似。本研究旨在探索FABP3及FABP4基因对秦川牛肉用新品系(以下简称“秦川肉牛”)肉质性状的影响,为秦川牛肉用选育提供理论基础。为了研究FABP3及FABP4基因对秦川肉牛肉质性状的影响,本试验首先采用生物信息学方法分析了FABP3及FABP4基因的生物学特征及生物进化信息。然后选取了饲养条件接近的571头18~36月龄的秦川肉牛作为试验材料,进行PCR扩增,采用直接测序法,运用DNAstar软件对测序结果进行对比分析,寻找突变位点;并与秦川肉牛肉质性状(背膘厚、眼肌面积和肌内脂肪含量)关联分析,以便为秦川肉牛选育改良工作筛选合适的分子标记,选择出具有优良性状的组合基因型,为秦川肉牛的科学选育提供理论支持。本研究的主要结果如下:1.FABP3基因序列编码133个氨基酸;FABP4基因序列编码132个氨基酸。生物信息学分析表明:该家族的基因序列在哺乳动物不同物种中具有较高的相似性。2.FABP3基因第3内含子上存在1个SNP位点g.60298CT;FABP4基因第1内含子上存在2个SNP位点g.2686AT和g.2834CG,第4外显子上存在1个SNP位点g.4220AG。只有g.2686AT存在两种基因型,分别是AA和AT基因型,TT基因型缺失。3.FABP3基因g.60298CT位点TT基因型个体均值显著高于CT和CC基因型(P0.05)。FABP4基因g.2834CG位点CC基因型肉质性状表现最优,眼肌面积均值比GG基因型均值高7.541cm2,差异极显著(P0.01);而肌内脂肪含量均值比GG基因型均值高0.254%,差异显著(P0.05)。4.FABP4基因g.4220AG位点TT基因型为优良基因型,在背膘厚方面,TT基因型显著高于CC基因型(P0.05)。5.FABP4基因3个SNP位点的优势单倍型组合为H1H1(ACA-ACA),其个体的眼肌面积极显著高于单倍型组合H3H3(P0.01);个体肌内脂肪含量均值极显著高于单倍型组合H3H7(P0.01)。因此,在本试验群体中,单倍型组合H1H1可被视为一种最为优秀的单倍型组合。综上,FABP3及FABP4基因与秦川肉牛肉质性状紧密关联,可作为影响肉质性状的有效候选基因用于标记辅助选择,有助于秦川肉牛定向高效选育和群体遗传改良。
[Abstract]:Fatty acid binding protein 3fat acid binding proteins 3 (FABP3) and fatty acid binding protein 4fat acid binding proteins 4 (FABP4) are important members of FABPs family. FABPs regulate fatty acid uptake and intracellular transport, although protein sequences vary greatly. The purpose of this study was to explore the effects of FABP3 and FABP4 genes on the meat quality traits of a new line of Qinchuan Beef (Qinchuan Beef cattle). In order to study the effects of FABP3 and FABP4 genes on meat quality of Qinchuan beef cattle, The biological characteristics and evolution information of FABP3 and FABP4 genes were analyzed by bioinformatics, and then 571 Qinchuan beef cattle (1836 months old) with similar feeding conditions were selected for PCR amplification. Direct sequencing method was used to compare and analyze the results of sequencing using DNAstar software to find mutation sites, and to analyze the correlation with meat quality traits (backfat thickness, eye muscle area and intramuscular fat content) of Qinchuan beef cattle. In order to select suitable molecular markers for the breeding and improvement of Qinchuan Beef cattle, and select combination genotypes with excellent traits, The main results of this study are as follows: 1. FABP3 gene sequence encodes 133 amino acids and FABP4 gene encodes 132 amino acids. Bioinformatics analysis shows that the gene sequence of this family is as follows: 1. 2. There is a SNP locus in intron 3 of FABP3 gene. 2. There are two SNP loci (g.2686AT and g.2834CG) on intron 1 of CTP4 gene of FABP3 gene, and one SNP locus (g.4220AGG) on exon 4 of FABP3 gene. Only g.2686AT in intron 1 of FABP3 gene. There are two genotypes, 3. The individual mean values of TT genotype at CT locus of FABP3 gene were significantly higher than those of CT genotypes and CC genotypes (P0.05N. FABP4 gene, g.2834CG), respectively. The mean area of eye muscle was 7.541 cm 2 higher than that of GG genotype, and the difference was very significant (P 0.01), while the mean value of intramuscular fat content was 0.254% higher than that of GG genotype. The difference was significant (P 0.05) .4.TT genotype at g.4220 AG of FABP4 gene was a good genotype. In terms of backfat thickness, the dominant haplotype combinations of the three SNP loci of the FABP4 gene were significantly higher than those of the CC genotype (P 0.05), and the area of the individual eye muscle was significantly higher than that of the haplotype combination H _ 3H _ 3P _ (0.01), and the mean value of intramuscular fat content was significantly higher than that of the haplotype combination H _ 3H _ 3H _ 3P _ (0.01). Haplotype combination H3H7, P0.01. therefore, In this population, haplotype H1 can be regarded as the best haplotype combination. FABP3 and FABP4 genes are closely related to the meat quality traits of Qinchuan Beef cattle, and can be used as effective candidate genes for marker-assisted selection of meat quality traits in Qinchuan Beef cattle. It is helpful to direct and efficient breeding and population genetic improvement of Qinchuan beef cattle.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S823

【参考文献】