鸡基质蛋白3基因的生物信息学分析

发布时间：2018-02-11 06:34

本文关键词： 鸡 matrin 克隆序列分析　出处：《中国家禽》2017年19期 　论文类型：期刊论文

【摘要】：根据GenBank已发表的鸡基质蛋白3(matrin 3)基因序列,设计合成一对特异性引物,以鸡胚成纤维细胞提取的总RNA反转录产物为模板,通过PCR扩增鸡matrin 3基因并进行克隆与生物信息学分析。测序结果表明:鸡matrin 3基因编码区全长为2 709 bp,共编码902个氨基酸,理论pI为5.79,分子质量约为100.71 ku,分子式为C_(4362)H_(6903)N_(1267)O_(1418)S_(29)。序列分析结果显示:鸡matrin 3蛋白有丰富的二级结构,以无规则卷曲和α-螺旋为主;该蛋白中包含两个锌指结构域和两个RNA识别基序,其中人、小鼠和野猪的4个功能结构域氨基酸位点相同,而在鸡matrin 3蛋白中存在多个氨基酸位点变异。核苷酸同源性及遗传进化分析发现:鸡与火鸡的matrin 3基因核苷酸同源性最高(为98.0%),并且与火鸡的亲缘关系最近。研究结果为进一步研究鸡matrin 3蛋白的生物学功能奠定了基础。
[Abstract]:A pair of specific primers were designed and synthesized according to the published chicken matrix protein 3matrin 3 gene sequence by GenBank. The total RNA reverse transcription product extracted from chicken embryo fibroblasts was used as template. Chicken matrin 3 gene was amplified by PCR, cloned and analyzed by bioinformatics. The result of sequencing showed that the encoding region of chicken matrin 3 gene was 2 709 BP, encoding 902 amino acids. The theoretical Pi is 5.79, the molecular weight is about 100.ku71, and the molecular formula is C# 4362HSP. The protein consists of two zinc finger domains and two RNA recognition motifs. The sequence analysis shows that the chicken matrin 3 protein has abundant secondary structure, mainly composed of irregular curls and 伪 -helix, and contains two zinc finger domains and two RNA recognition motifs, the sequence analysis of which shows that the chicken matrin 3 protein has abundant secondary structure and is characterized by irregular curl and 伪 -helix, and contains two zinc finger domains and two RNA recognition motifs. The amino acid loci of four functional domains of human, mouse and wild boar are the same. The nucleotide homology and genetic evolution analysis showed that the nucleotide homology of matrin 3 gene between chicken and turkey was the highest (98.0%), and the relationship between chicken and turkey was close. The results laid a foundation for further study on the biological function of chicken matrin 3 protein.
【作者单位】：贵州大学动物科学学院;贵州大学高原山地动物遗传育种与繁殖教育部重点实验室;
【基金】：国家自然科学基金项目(31502074) 贵州省科学技术基金项目(黔科合J字[2015]2054号) 贵州大学引进人才科研项目(贵大人基合字[2014]10号)
【分类号】：S831

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