不同海拔猪种EPO基因核苷酸多态性研究

发布时间：2018-02-11 19:27

本文关键词： 猪 EPO 多态性　出处：《基因组学与应用生物学》2017年03期 　论文类型：期刊论文

【摘要】：青藏高原低氧胁迫响应下,藏猪在长期进化过程中形成了独特的高原低氧适应性。本研究对藏猪(合作猪,甘孜藏猪,迪庆猪,西藏藏猪)、八眉猪、烟台黑猪及长白猪、杜洛克猪、大白猪促红细胞生成素(erythropoietin,EPO)基因全序进行了测定分析。所得数据表明:9个猪群体的37个核苷酸序列共得到14个单倍型,25个变异位点包括8个转换,3个颠换和一个连续14 bp的缺失,其中有义突变3个;引入猪种仅分布在两个单倍型H1和H2中,我国地方猪种则分布在13个单倍型中;分析发现藏猪"TTGGGAAGTCTCAT"的一个序列片段缺失,这一特征在低海拔群体中不存在。EPO基因编码的194个氨基酸中发现3个突变位点,变异后的单倍型及氨基酸序列在藏猪中占优势。因此,在适应高寒低氧环境的生理学和分子学机制方面,藏猪EPO基因在长期进化过程中可能起着关键性遗传作用。
[Abstract]:In response to hypoxia stress in the Qinghai-Tibet Plateau, Tibetan pigs developed a unique adaptation to high altitude hypoxia during their long-term evolution. In this study, Tibetan pigs (Hehe pig, Ganzi Tibetan pig, Diqing pig, Tibetan Tibetan pig, Bamei pig, Yantai black pig and Landrace pig) were studied. Duroc pigs, The total sequence of erythropoietin erythropoietin (EPO) gene in large white pigs was determined and analyzed. The data showed that there were 14 haplotypes in 37 nucleotide sequences of 9 pig populations, 25 mutation sites including 8 conversions, 3 transversions and a continuous deletion of 14 BP. Among them, there were 3 sense mutants, only two haplotypes H1 and H2 were introduced into pig breeds, and 13 haplotypes were found in native pig breeds in China, and a sequence deletion of "TTGGGAAGTCTCAT" in Tibetan pigs was found by analysis. Three mutation sites were found in 194 amino acids encoded by EPO gene in low altitude population, and the haplotypes and amino acid sequences were dominant in Tibetan pigs. The EPO gene may play a key genetic role in the long-term evolution of Tibetan pigs in the physiological and molecular mechanisms of adaptation to alpine and hypoxic environments.
【作者单位】：甘肃农业大学动物科学技术学院;
【基金】：甘肃农业大学青年研究生指导教师扶持基金(GAU-QNDS-201401)资助
【分类号】：S828

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