展示H5、H7、H9血凝素和N1神经氨酸酶的病毒样颗粒引发鸡对异源禽流感病毒保护性免疫的研究

发布时间：2018-02-11 23:26

本文关键词： 禽流感流感重组 VLP 疫苗病毒样颗粒　出处：《中国畜牧兽医》2017年01期 　论文类型：期刊论文

【摘要】：在野生禽类中流行的禽流感病毒(AIV)对公众健康构成严重威胁,迫切需要针对多种禽流感亚型的人用和兽用疫苗。本试验制备了针对H5、H7和H9 3种AIV亚型抗原的病毒样颗粒(VLPs)(分别源自H5N1、H7N3和H9N2病毒)。VLPs还含有流感N1神经氨酸酶和逆转录病毒gag蛋白。利用杆状病毒表达系统制备H5/H7/H9/N1/gag VLP。本试验测定了其包括血凝素和神经氨酸酶活性在内的生化特性、功能和抗原特性。在鸡禽流感攻击模型中进一步评估VLPs的安全性、免疫原性和针对异源AIV(包括H5N2、H7N3和H9N2亚型AIV)的保护功效。结果显示,接种疫苗的禽类在H5N2和H7N3高致病性AIV的攻击中未出现死亡,而对照组全部死亡。用低致病性H9N2亚型AIV攻毒后也可检测到免疫应答,结果表明H5/H7/H9/N1/gag VLP为具有广泛免疫保护作用的禽流感疫苗的开发提供了新方法。
[Abstract]:Avian influenza virus (AIV), which is prevalent in wild birds, poses a serious threat to public health. There is an urgent need for human and veterinary vaccines against a variety of avian influenza subtypes. In this study, viroid particles targeting H5H7 and H9 AIV subtype antigens were prepared (derived from H5N1 N1H7N3 and H9N2 viruses, respectively. VLPs also contain influenza N1 neuraminidase and reverse neuraminidase). Transcription virus gag protein. Use baculovirus expression system to prepare H5N1 / H7 / H9 / N1 / gag VLP. this experiment has determined its biochemical characteristics, including hemagglutinin and neuraminidase activity. Function and antigenic properties. Further evaluation of the safety, immunogenicity and protection of VLPs against allogeneic AIVs (including H5N1 N2H7N3 and H9N2 subtypes AIVs) in chicken avian influenza attack models. The vaccinated birds did not die in attack of H5N1 N2 and H7N3 high pathogenic AIV, but all of the control group died. The immune response was also detected after attack with low pathogenic H9N2 subtype AIV. The results show that H5N1 / H _ 7 / H _ 9 / N _ 1 / gag VLP provides a new method for the development of avian influenza vaccine with extensive immune protection.
【分类号】：S852.4

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