策勒黑羊卵巢总RNA的提取

发布时间：2018-02-12 01:46

本文关键词： 异硫氰酸胍法卵巢 RNA提取策勒黑羊遗传资源　出处：《黑龙江畜牧兽医》2017年08期 　论文类型：期刊论文

【摘要】：策勒黑羊具有全年发情、多胎的特点,是新疆策勒县特有的优良绵羊品种。为研究与保护策勒黑羊遗传资源,为后续开展逆转录酶聚合酶链式反应(RT-PCR)、斑点杂交试验和构建c DNA文库试验等提供条件,试验采用经典异硫氰酸胍(Trizol)法提取策勒黑羊卵巢总RNA,通过核酸蛋白仪测定所提取RNA在不同波长下的吸光度值,计算RNA的提取量与纯度,并利用琼脂糖凝胶电泳显示提取结果和验证RNA的完整性。结果表明:采用Trizol法提取得到的策勒黑羊卵巢RNA的浓度、纯度与完整性均可达到后续试验的要求,可为后续策勒黑羊种质资源保护和利用等生物学试验的开展提供参考依据。
[Abstract]:Cele Black Sheep has the characteristics of full year estrus and multiple births, and is a unique fine sheep breed in Cele County, Xinjiang. In order to study and protect the genetic resources of Cele Black Sheep, To provide conditions for reverse transcriptase polymerase chain reaction reverse transcriptase polymerase chain reaction reverse transcriptase polymerase chain reaction reverse transcriptase polymerase chain reaction RT-PCRN, dot blot hybridization and construction of c DNA library. In this experiment, the classical guanidine thiocyanate (Trizol) method was used to extract the total RNA from the ovary of the black sheep. The absorbance of the extracted RNA at different wavelengths was determined by nucleic acid protein analyzer, and the extraction amount and purity of RNA were calculated. Agarose gel electrophoresis was used to show the extraction results and verify the integrity of RNA. The results showed that the concentration, purity and integrity of ovarian RNA extracted by Trizol method could meet the requirements of subsequent tests. It can provide reference for the following biological experiments such as conservation and utilization of Cele black sheep germplasm resources.
【作者单位】：塔里木大学动物科学学院;塔里木大学生命科学学院;
【基金】：国家自然科学基金项目(31460597) 新疆生产建设兵团塔里木畜牧科技重点实验室开放课题(HS201401) 塔里木大学科研项目(TDZKCX201401)
【分类号】：S826

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