绵羊KITLG基因的表达及其多态性与产羔性状关联分析

发布时间：2018-02-12 12:35

本文关键词： KITLG基因单核苷酸多态性产羔数基因表达绵羊　出处：《农业生物技术学报》2017年06期 　论文类型：期刊论文

【摘要】：繁殖性状作为绵羊重要的经济性状,其分子机理的研究极为重要。绵羊(Ovis aries)繁殖性能受到下丘脑-垂体-性腺生殖轴的调控,同时也受到许多的生长因子的调节,KIT的配体KITLG(KIT LIGAND,KL或KitL)作为重要的生长调节因子在卵泡发育中起很重要的作用。为了解绵羊KITLG基因表达特征、多态性及其与产羔性状的关联性,本研究利用Real-time PCR分析了KITLG基因在湖羊下丘脑、垂体、卵巢、心脏、肌肉、肺、肾脏、肝脏、脾脏、十二指肠、脂肪和瘤胃等组织中的表达水平,同时采用限制性长度片段多态性(restriction fragment length polymorphism,RFLP)技术检测了KITLG基因在湖羊和小尾寒羊群体中的多态性及其与产羔性状的关联性。结果表明,绵羊KITLG基因在上述检测的12个组织中均有表达,其中在卵巢中的表达量较高。通过PCR-RFLP分析,在第2和第5内含子上检测到g.38473CT和g.47468CT 2个突变位点。在小尾寒羊和湖羊群体中检测到的g.38473CT位点上均有CC(优势基因型)和CT两种基因型,其中C为优势等位基因;湖羊群体的基因杂合度(heterozygosity,He)为0.89,纯合度(homozygosity,Ho)为0.11,有效等位基因数(effective allele number,Ne)和多态信息含量(polymorphic information content,PIC)分别为1.31和0.11;在小尾寒羊中分别为0.80、0.20、1.10和0.16,并且在该突变位点,两个群体均处于Hardy-Weinberg平衡状态。在g.47468CT突变位点上检测到CT和TT(优势基因型)两种基因型,其中优势等位基因为T;在此位点湖羊群体中He、Ho、Ne及PIC分别为0.95、0.05、1.06、0.05;小尾寒羊群体中分别为0.91、0.09、1.10和0.09。两个群体在该位点均处于Hardy-Weinberg平衡状态。多态位点与绵羊产羔数的关联性分析表明,KITLG基因g.47468CT位点与小尾寒羊产羔数显著相关(P0.05)。本研究结果揭示了KITLG基因在绵羊卵巢中的表达量较高,且g.47468CT位点与绵羊产羔数显著相关,可作为产羔性状相关的候选基因。
[Abstract]:As an important economic trait in sheep, the study of its molecular mechanism is very important. The reproductive performance of Ovis aries is regulated by the hypothalamus-pituitary-gonad reproductive axis. The ligand KITLG(KIT LIGDANDKL or Kit, which is also regulated by many growth factors, plays an important role in follicular development. In order to understand the characteristics of KITLG gene expression, polymorphism and its association with lambing traits in sheep, Real-time PCR was used to analyze the expression of KITLG gene in the hypothalamus, pituitary, ovary, heart, muscle, lung, kidney, liver, spleen, duodenum, fat and rumen of Hu sheep. At the same time, restriction restriction fragment length polymorphism was used to detect the polymorphism of KITLG gene in Hu sheep and small tail Han sheep population and its association with lambing traits. Sheep KITLG gene was expressed in 12 tissues, especially in ovary. PCR-RFLP analysis was used to analyze the expression of sheep KITLG gene. Two mutation loci, g.38473CT and g.47468CT, were detected on intron 2 and intron 5. Among them, there were two genotypes of CC-C and CT on the locus of g.38473CT detected in small tail Han sheep and Hu sheep, in which C was the dominant allele. Heterozygosity He0.89, homozygosity of homozygosity 0.11, effective number of alleles and polymorphic information contentPICs were 1.31 and 0.11in Hu sheep, 0.800.201.10 and 0.16in small tail Han sheep, respectively. Both populations were in Hardy-Weinberg equilibrium. Ct and TT2 genotypes were detected at the mutation locus of g.47468CT. Among them, the dominant alleles were T, the PIC and the PIC of the Hu sheep population were 0.950.05 and 1.060.05, respectively, and those of the small tail Han sheep were 0.91 ~ 0.09 ~ 1.10 and 0.09, respectively. Both populations were in the Hardy-Weinberg equilibrium state at this locus, and the correlation between the polymorphic loci and sheep litter size was also observed. The results showed that there was a significant correlation between G47468CT locus of KITLG gene and litter size of small tail Han sheep. The results showed that the expression of KITLG gene was high in ovaries of sheep. Moreover, the locus of g.47468CT was significantly correlated with sheep litter size and could be used as a candidate gene for lambing traits.
【作者单位】：甘肃农业大学动物科技学院;青海大学农牧学院;兰州大学草地农业科技学院;甘肃省肉羊繁育生物技术工程实验室民勤陇原中天生物工程有限公司;
【基金】：国家自然科学基金项目(No.31472072) 国家肉羊现代肉羊产业技术体系建设专项(CARS-39)
【分类号】：S826

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