烟台黑猪SLA-DRA基因编码区多态性及生物信息学分析

发布时间：2018-02-13 04:06

本文关键词： SLA-DRA基因烟台黑猪编码区多态性蛋白质结构分子进化树　出处：《华北农学报》2017年01期 　论文类型：期刊论文

【摘要】：为了探究烟台黑猪SLA-DRA基因多态性,预测其蛋白质的结构并分析功能特征,采用PCR-SSCP、克隆测序和生物信息学等方法对SLA-DRA基因4个外显子的多态性、蛋白质的理化性质、结构域、蛋白质的空间结构和功能进行了预测和分析。结果显示:SLA-DRA基因exon 1、exon 2和exon 4共检测到11个SNPs,导致7个氨基酸发生变异,exon 3没有突变产生,exon 4的多态性最丰富。SLA-DRA基因编码区759 bp核苷酸共编码252个氨基酸和1个终止子。SLA-DRA基因蛋白质是一种偏酸性、不稳定、亲水性蛋白质,其二级结构是混合型,其中无规则卷曲最多。该蛋白质含有2个免疫球蛋白结合域、2个N-糖基化位点和10个磷酸化位点。功能分析显示:SLA-DRA基因蛋白质在信号转导、受体、胁迫应答、免疫应答、生长因子等方面的几率均较高。研究结果可为猪的抗病分子育种候选基因筛选提供理论依据。
[Abstract]:In order to investigate the polymorphism of SLA-DRA gene in Yantai Black Pig, predict its protein structure and analyze its functional characteristics, PCR-SSCP, clone sequencing and bioinformatics were used to analyze the polymorphism, physicochemical properties and domain of four exons of SLA-DRA gene. The spatial structure and function of protein were predicted and analyzed. The results showed that 11 SNPs were detected in exon 1 exon2 and exon 4 of the SLA-DRA gene, which resulted in 7 amino acids mutated without mutation to produce the polymorphism of SLA-DRA 4. The 759 BP nucleotide encoding 252 amino acids and one Terminator SLA-DRA gene protein is a partial acid. Unstable, hydrophilic proteins with mixed secondary structures, The protein contains two immunoglobulin binding domains, two N-glycosylation sites and 10 phosphorylation sites. The results can provide theoretical basis for screening candidate genes for molecular breeding of disease resistance in pigs.
【作者单位】：甘肃农业大学动物科学技术学院;河南省漯河市畜牧局;甘肃省现代养猪工程技术研究中心;
【基金】：甘肃省农牧厅生物技术专项(GNSW-2008-04) 甘肃省科技重大专项(092NKDA036) 甘肃省科技支撑计划项目(0804NKCA065) 国家自然基金项目(31660646)
【分类号】：S828

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