利用高通量测序技术分析川中黑山羊瘤胃纤毛虫种群结构

发布时间：2018-02-15 01:51

本文关键词： 川中黑山羊瘤胃纤毛虫种群结构多样性 MiSeq测序　出处：《动物营养学报》2017年05期 　论文类型：期刊论文

【摘要】：本试验旨在应用高通量测序技术研究川中黑山羊瘤胃纤毛虫种群结构。选取3只4月龄川中黑山羊[体重(15.53±0.21)kg],正常饲喂20 d后采集瘤胃液(A)样品,间隔40 d后再次采集瘤胃液(F)样品,提取样品总DNA后,扩增真核生物18S rRNA V4区,扩增产物使用Illumina MiSeq平台测序。结果表明:1)共获得高质量有效序列242 321条,聚类后得1 650个操作分类单位(OTU)。2)A样品、F样品在alpha多样性Chao、ACE、Shannon和Simpson指数上,差异不显著(P0.05)。3)在纲水平分类上,2个时间点样品相对丰度最高的均为纤毛门,侧口纲(A样品为46.0%、F样品为44.7%),2个样品的相对丰度差异不显著(P0.05)。4)在科水平分类上,A样品优势科为头毛科(31.8%),其次为均毛科(14.2%);F样品优势科为头毛科(42.8%);并且F样品头毛科相对丰度显著高于A样品(P0.05),A样品均毛科相对丰度显著高于F样品(P0.05)。5)在属水平分类上,A样品与F样品相对丰度最高的属均为多加多泡双毛属(A样品为20.9%、F样品为25.4%),无显著差异(P0.05);存在显著性差异的属是均毛属、头毛属、腹甲双毛属、刺甲双毛属相对丰度,其中A样品均毛属(14.1%vs.1.9%)、腹甲双毛属相对丰度(2.8%vs.1.5%)显著高于F样品(P0.05),而头毛属(6.7%vs.12.5%)、刺甲双毛属相对丰度(0.3%vs.2.5%)显著低于F样品(P0.05)。本试验结果表明,幼龄川中黑山羊瘤胃液纤毛虫相对丰度最高的种属为多加多泡双毛属,瘤胃中还有许多未被分类鉴定且相对丰度较高的真核生物,需要进一步研究
[Abstract]:The purpose of this study was to study the population structure of rumen ciliates of black goats in Chuanzhong by high-throughput sequencing. Three four-month-old black goats [weight 15.53 卤0.21kg] were collected from rumen fluid after 20 days of normal feeding. After 40 days' interval, the rumen fluid F) samples were collected again, the total DNA of the sample was extracted, the 18 S rRNA V4 region of eukaryote was amplified, and the amplified product was sequenced using Illumina MiSeq platform. The results showed that the high quality effective sequence 242321 was obtained. After clustering, 1 650 samples of OTU. 2A and F were not significantly different in the alpha diversity of ACEN Shannon and Simpson. 3) in class level classification, the highest relative abundance of samples at two time points was phylum ciliata. The relative abundance of the two samples was 44.70.The relative abundance of the two samples was not significant (P0.05n.4) in the level classification, the dominant family of sample A was 31.8%, the next was the dominant family of Cephalopodiaceae 14.2%, the dominant family of F sample was 42.8%; and the dominant family of the F sample was Cephalopodiaceae 42.8%, and the dominant family of sample A was Cephalopodiaceae 31.8%, and the dominant family of F sample was Cephalopodiaceae 42.8%. The relative abundance of P0.05A sample is significantly higher than that of F sample P0.05U 路5) the highest relative abundance between A sample and F sample is 20.9% F sample, no significant difference is found in the taxonomy of genus A and F sample in the taxonomy of the genus P0.05. 5) the relative abundance of P0.05A sample is significantly higher than that of sample F sample P0.05. 5) on the taxonomic level, the highest relative abundance of samples A and F is 20.9U / F, with no significant difference. There was significant difference in the genus P0.05, and the genus in which there was a significant difference in the genus P0.05. The relative abundance of the genus Cephalocephala, the genus Bimodendron and the genus Bimodendron, The relative abundance of P0.05A was significantly higher than that of F sample P0.05, while the relative abundance of Cephalocephala 6.7vs.12.5was significantly lower than that of F sample P0.055.The results showed that the relative abundance of P0.05 was significantly lower than that of F sample P0.05. The highest relative abundance of rumen ciliates in young Sichuan black goats is the genus Polyvesicular, and there are many eukaryotes in the rumen which have not been classified and identified and have relatively high abundance. It needs further study.
【作者单位】：四川农业大学动物医学院动物疫病与人类健康四川省重点实验室环境公害与动物疾病四川省高校重点实验室;
【基金】：“长江学者和创新团队发展计划”创新团队项目(IRT0848) 四川农业大学双支计划(03572070)
【分类号】：S827

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