抗氧化剂对马精子低温及冷冻保存效果的影响

发布时间：2018-02-15 04:52

本文关键词： 抗氧化剂精液冷冻保存马　出处：《中国畜牧兽医》2017年07期 　论文类型：期刊论文

【摘要】：试验旨在研究不同抗氧化剂对马精子低温保存及冷冻效果的影响。选取6匹英纯血种公马作为试验动物,以INRA82液作为基础稀释液(对照组),分别添加谷氨酰胺(0.015g/mL)、甘氨酸(0.019g/mL)、半胱氨酸(0.024g/mL)、甲硫氨酸(0.015g/mL)、牛磺酸(0.063g/mL)、维生素C(0.4mg/mL)、维生素E(0.5mg/mL)、褪黑素(0.001mg/mL)制备成含有不同抗氧化剂的低温保存和冷冻保存稀释液,将浓缩处理后的马精子分别置于上述稀释液中保存或冷冻,检测低温保存48h后精子运动参数,评价抗氧化剂对精子低温保存的影响;检测精子冻融后运动参数、精子质膜完整性、线粒体膜电势及顶体完整性,评价抗氧化剂对精子冷冻效果的影响。结果表明,精子低温保存48h后,添加牛磺酸组活精子比例和前向运动精子比例显著高于对照组(P0.05),添加维生素C组前向运动精子比例显著高于对照组(P0.05)。冷冻解冻后结果表明,添加不同抗氧化剂没有改善精子冻融后活精子比例和前向运动精子比例,但添加甲硫氨酸显著延长了精子体外存活能力(P0.05);添加不同抗氧化剂精子质膜完整性与对照组间无显著差异(P0.05),但甲硫氨酸和甘氨酸组有高于对照组的趋势;添加维生素E和甲硫氨酸组精子线粒体膜电势显著高于对照组(P0.05),不同抗氧化剂组精子顶体完整性与对照组间均无显著差异(P0.05)。结果提示,低温保存时添加牛磺酸和维生素C可以提高精液低温保存效果;冷冻时添加甲硫氨酸能提高精子质膜完整性和线粒体膜电势,且能够延长精子冻融后的存活时间。
[Abstract]:The purpose of the experiment was to study the effects of different antioxidants on cryopreservation and cryopreservation of horse spermatozoa. Using INRA82 solution as basic diluent (control group, adding 0.015 g / mL of glutamine, 0.019 g / mL of glycine, 0.024 g / mL of cysteine, 0.015 g / mL of methionine, 0.063 g / mL of taurine, 0.4 mg / mL of vitamin C, 0.5 mg / mL of vitamin E, 0.001 mg / mL of melatonin). And cryopreservation diluents, The equine spermatozoa after concentrated treatment were stored or frozen in the above diluent, the sperm motility parameters were measured after 48 hours of cryopreservation, the effects of antioxidants on sperm cryopreservation were evaluated, and the sperm motility parameters after freezing and thawing were detected. The sperm plasma membrane integrity, mitochondrial membrane potential and acrosome integrity were evaluated to evaluate the effects of antioxidants on sperm cryopreservation. The results showed that the sperm was cryopreserved for 48 hours. The percentage of live sperm and forward motile sperm in taurine group was significantly higher than that in control group (P 0.05), and that in vitamin C group was significantly higher than that in control group (P 0.05). The addition of different antioxidants did not improve the percentage of live sperm and forward motile sperm after freezing and thawing. Addition of methionine significantly prolonged the viability of spermatozoa in vitro, and the integrity of plasma membrane of spermatozoa supplemented with different antioxidants was not significantly different from that of the control group (P 0.05), but there was a higher trend in methionine and glycine groups than in the control group. The mitochondrial potential of sperm in vitamin E and methionine groups was significantly higher than that in control group (P 0.05), but there was no significant difference in acrosome integrity between different antioxidant groups and control group. The results suggested that the mitochondrial membrane potential of spermatozoa in vitamin E and methionine groups was significantly higher than that in control group. The addition of taurine and vitamin C during cryopreservation can improve the cryopreservation effect of semen, and the addition of methionine can improve the integrity of sperm plasma membrane and the potential of mitochondrial membrane, and prolong the survival time after freezing and thawing.
【作者单位】：新疆农业大学动物科学学院;新疆畜牧科学院畜牧研究所;新疆昭苏县畜牧兽医局;
【基金】：国家自然科学基金:马精子冷冻保存方法及精子冻融损伤机制的研究(31360550);法国速步马及培育关键技术引进实验研究(2014DFA31370)
【分类号】：S821

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