GH基因第3内含子多态性及与生长性状的相关性

发布时间：2018-02-17 00:53

本文关键词： 甘肃高山细毛羊 GH基因第3内含子多态性日增重　出处：《甘肃农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：本试验采用PCR-SSCP技术检测甘肃高山细毛羊及其与特克赛尔羊、邦德羊和澳洲美利奴羊的杂交后代的GH基因第3内含子多态性,分析GH基因第3内含子突变对羔羊初生重、3月龄重、断奶重及日增重的影响,为绵羊的经济性状相关基因研究提供基础资料。主要研究结果如下:甘肃高山细毛羊及其杂种羔羊GH基因第3内含子多态性与生长性状的相关性:通过检测317只不同群体绵羊GH基因第3内含子多态性,结合生产数据分析不同基因型与初生重、3月龄重、断奶重和日增重的相关性。结果表明,甘肃高山细毛羊及其杂种羔羊GH基因第3内含子第98bp处有一个CT突变,共有AA、AB和BB三种基因型,甘肃高山细毛羊和杂种羔羊的GH基因第3内含子PIC值均介于0.25-0.5之间,为中度多态。其中BB型为优势基因型。甘肃高山细毛羊不同基因型与初生重间关联度不明显(P0.05),BB型甘肃高山细毛羊3月龄重和断奶重高于AA型(P0.05),AA型甘肃高山细毛羊日增重与BB型相关(P0.05)。特甘细BB型的初生重、断奶重及日增重均高于AA型特甘细羊(P0.01)。邦甘细羊、澳甘细羊不同基因型与体重及日增重均无相关性(P0.05)。综合以上结果GH基因第3内含子等位基因B对甘肃高山细毛羊生长性状可能产生有利影响,初步推断可作为甘高细和特甘细羊生长标记候选基。
[Abstract]:PCR-SSCP technique was used to detect the intron 3 polymorphism of GH gene in Gansu Alpine fine wool sheep and its progeny with Teksell sheep, Bond sheep and Australian Merino sheep. Effects of weaning and daily gain, The main results are as follows: the relationship between intron 3 polymorphism of GH gene and growth traits in Gansu alpine fine wool sheep and its hybrid lambs: 317 sheep were tested. The polymorphism of intron 3 of GH gene in different populations of sheep, Combined with production data, the correlation between different genotypes and birth weight, weaning weight and daily gain was analyzed. The results showed that there was a CT mutation at 98bp in intron 3 of GH gene of Gansu Alpine fine wool sheep and its hybrid lambs. There were three genotypes of AA AB and BB. The PIC values of intron 3 of GH gene in Gansu Alpine fine wool sheep and hybrid lamb were between 0.25-0.5. The correlation between different genotypes and birth weight of Gansu alpine fine wool sheep was not obvious (P 0.05) and weaning weight of Gansu alpine fine wool sheep was higher than that of Gansu alpine fine wool sheep of AA type (P 0.05). Daily gain was related to BB type (P0.05N). The weaning weight and daily gain were higher than that of AA type special sweet fine sheep (P0.01). There was no correlation between different genotypes and body weight and daily gain of Australian sweet sheep (P 0.05). Combined with the above results, allele B of intron 3 of GH gene may have a beneficial effect on growth traits of Gansu alpine fine wool sheep. It was preliminarily deduced that it could be used as candidate for growth marker of tall sweet sheep and super sweet fine sheep.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S826

【共引文献】