维生素A及蛋氨酸锌对断奶仔猪血液抗氧化指标、免疫功能及肠道功能影响

发布时间：2018-02-20 06:57

本文关键词： 维生素A 蛋氨酸锌断奶仔猪抗氧化指标免疫功能肠道功能　出处：《黑龙江八一农垦大学》2016年硕士论文　论文类型：学位论文

【摘要】：为研究日粮中不同水平维生素A(VA)与蛋氨酸锌(Zn Met)对断奶仔猪生长性能、血液生化指标和抗氧化指标、免疫功能及肠道功能的影响,试验采用两因素三水平析因设计,选择81头28日龄断奶、体重为(7.94±0.62)kg的健康杜长大三元仔猪,按体重和性别随机分为9个处理组,每个处理3个重复,分别饲喂含不同剂量VA(0、3 000、6 000 IU/kg)和Zn Met(0、300、400 mg/kg)的日粮。试验结果表明:(1)在日粮中添加3 000 IU/kg VA、300 mg/kg Zn Met即可满足35-56日龄断奶仔猪生长性能的需要。更高水平的VA与Zn Met能更好的促进仔猪生长,但与上述水平的影响效果不显著。经过一段时间饲喂后,VA与Zn Met互作效应出现显著。(2)6 000 IU/kg VA对断奶仔猪影响效果略优于3 000 IU/kg VA,6 000 IU/kg在28日龄断奶仔猪的VA正常使用量范围内;400 mg/kg Zn Met抑制了试验后期仔猪IL-2的分泌和试验前期仔猪IL-4的分泌;VA与Zn Met互作效应显著。(3)从血液生化指标来看,两种水平VA均能显著提高AKP活性,降低UN含量,对CHO、TG含量和GPT、GOT活性影响均不显著;两水平Zn Met均能显著降低TG、UN含量,提高AKP活性,对CHO含量和GPT、GOT活性影响均不显著;对UN指标来说,VA同Zn Met互作效应显著。从血清抗氧化指标考虑,低日龄所需VA和Zn Met的量分别为6 000 IU/kg和300 mg/kg;高日龄所需VA和Zn Met的量分别为6 000 IU/kg和400mg/kg;对MDA指标来说,VA同Zn Met互作效应极显著。(4)VA与Zn Met均能显著提高断奶仔猪小肠绒毛高度、绒毛宽度并能显著降低小肠隐窝深度。VA与Zn Met对提高断奶仔猪小肠二糖酶活性都发挥了促进作用,且VA所产生的作用更大,影响效果更显著。
[Abstract]:In order to study the effects of different levels of vitamin A (VA) and zinc methionine (Zn) on growth performance, blood biochemical index and antioxidant index, immune function and intestinal function of weaned piglets, two factors and three levels of factorial design were used in the experiment. A total of 81 28 day weaned healthy Ducang Sanyuan piglets, weighing 7.94 卤0.62kg, were randomly divided into 9 treatment groups according to their body weight and sex, with 3 replicates in each treatment. The diets were fed with different doses of VA0 3 000 U / kg and Zn Meto 300 mg / kg respectively. The results showed that the addition of 3 000 IU/kg VA0 300 mg/kg Zn Met to the diets could meet the requirement of growth performance of weaned piglets at 35 to 56 days of age, and higher levels of VA and Zn Met energy could be obtained. Better promote the growth of piglets, But the effect with the above level was not significant. After feeding for a period of time, the effect of the interaction between VA and Zn Met was significant. The effect of IU/kg VA on weaning piglets was slightly better than that of 3 000 IU/kg VA6 000 IU/kg at 28 days old weaning piglets. In the range of dosage, 400 mg/kg Zn Met inhibited the secretion of IL-2 in piglets in the later stage of the experiment and the interaction effect of IL-4 secretion with Zn Met in the early stage of the experiment. Both levels of VA could significantly increase the activity of AKP, decrease the content of UN, and have no significant effect on the content of Cho, TG and got, but Zn Met at both levels could significantly decrease the content of AKP and increase the activity of AKP, but had no significant effect on the content of CHO and the activity of GPTG. The interaction effect between VA and Zn Met was significant for UN indexes. The amounts of VA and Zn Met were 6 000 IU/kg and 300 mg / kg for low age and 6 000 IU/kg and 400 mg / kg for Zn Met at high age, respectively. For MDA, the interaction effect between VA and Zn Met was very significant. Villi width and intestinal crypt depth decreased significantly. VA and Zn Met promoted the increase of small intestinal dismutase activity in weaned piglets, and VA produced more significant effects.
【学位授予单位】：黑龙江八一农垦大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S828.5

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