黑龙江某规模化猪场2015-2016年伪狂犬病血清学调查及三种疫苗免疫效果研究

发布时间：2018-02-20 16:15

本文关键词： 伪狂犬病抗体阳性率 g E g B 血清学调查　出处：《黑龙江八一农垦大学》2017年硕士论文　论文类型：学位论文

【摘要】：猪伪狂犬病是由伪狂犬病毒引起的以繁殖障碍、脑脊髓炎、呼吸道疾病等症状为主的急性传染病。目前黑龙江省伪狂犬病流行现状呈上升趋势,严重制约养猪业发展。本试验通过血清学调查猪伪狂犬病抗体在规模化猪场的变化规律,同时开展不同毒株的三种疫苗在伪狂犬病阴性猪场和阳性猪场的免疫效果对比试验,以期为猪场制定合理的免疫程序和选择疫苗提供理论依据和试验参考。本试验对黑龙江省某存栏1 200头母猪猪场在2015年9月-2016年10月分三次采集不同群体样本血清进行猪伪狂犬病g E抗体及g B抗体检测,同时针对断奶前后不同日龄猪群进行猪伪狂犬病g B抗体检测以确定母源及疫苗抗体的消减规律。结果显示:2015年9月采集336头猪群中g E抗体阳性猪208头,阳性率61.90%,g B抗体阳性猪为314头,阳性率为93.45%;2016年4月采集200头猪群中g E抗体阳性猪120头,阳性率60.00%,g B抗体阳性猪为174头,阳性率为87.00%;2016年10月采集187头猪群中g E抗体阳性猪为92头,阳性率为49.20%,g B抗体阳性猪为141头,阳性率为75.40%。其中2016年4月对猪场35日龄、45日龄、70日龄猪群检测g B抗体阳性率分别为70.00%、50.00%、75.00%,45日龄仔猪g B抗体阳性率为50.00%,为适宜的首免日龄,结果显示该猪场伪狂犬病感染情况较为严重,但野毒抗体呈下降趋势。本次血清学调查结果为猪场开展伪狂犬病的防控,制定符合本场情况的免疫程序提供了依据。根据上述试验结果选取猪伪狂犬病阴性场和阳性场进行三种不同疫苗免疫效果对比试验,选取35日龄的60头健康仔猪随机分为3组分别免疫不同毒株的三种疫苗,分别为A疫苗(国产SA毒株)、B疫苗(国产Bartha毒株)、C疫苗(进口Bartha毒株),于首免前1天、二免前1天、二免28天后分别采集血清检测猪伪狂犬病野毒g E抗体和疫苗g B抗体水平。结果显示:阴性场猪伪狂犬病g E抗体阳性率为0%,说明猪场没有受到野毒感染。二免前1天,A疫苗、C疫苗抗体阳性率在90%以上,B疫苗抗体阳性率为50%,二免后28天,A、C疫苗抗体阳性率为100%,高于B疫苗抗体阳性率90.00%。阳性猪场检测结果显示首免前1天g E、g B抗体阳性率差异不显著(P0.05),二免前1天检测猪伪狂犬g E抗体阳性率分别为50%、45%、40%,g B抗体阳性率分别为50%、60%、65%;二免后28天检测猪伪狂犬g E抗体阳性率分别为35%、40%、35%,猪伪狂犬g B抗体分别为75%、70%、80%。本次疫苗对比试验为猪场选择符合本场的疫苗提供了数据依据。综上所述,黑龙江某1 200头规模化猪场伪狂犬病野毒感染呈下降趋势,根据母源抗体消减规律,最佳首免日龄为45日龄。对比疫苗试验结果显示三种疫苗均能在二免后产生有效抗体,首免后国产SA疫苗与进口Bartha疫苗免疫效果优于国产Bartha疫苗,国产SA疫苗与进口Bartha疫苗差异不大。
[Abstract]:Porcine pseudorabies is an acute infectious disease caused by pseudorabies virus, which is characterized by reproductive disorders, encephalomyelitis, respiratory diseases, etc. At present, the prevalence of pseudorabies in Heilongjiang Province is on the rise. The changes of pseudorabies antibody in large scale pig farms were investigated by serology. At the same time, three kinds of vaccines of different strains were tested in the pseudorabies negative pig farm and the positive pig farm. The aim of this study was to provide theoretical basis and experimental reference for the establishment of reasonable immunization procedures and vaccine selection in pig farms. This experiment was used to collect blood samples from different populations three times from September 2015 to October 2016 in a pig farm in Heilongjiang province. The antibody against porcine pseudorabies virus (GE) and the antibody against porcine pseudorabies (GB) were detected. At the same time, porcine pseudorabies g B antibody was detected before and after weaning to determine the subtractive rule of maternal and vaccine antibodies. The results showed that: in September 2015, 208 pigs were positive for GE antibody. In April 2016, 120 of 200 pigs were positive for GE antibody, and the positive rate was 60.000.The positive rate was 60.00g B antibody positive pigs, and the positive rate was 93.455.In April 2016, the positive rate of g E antibody positive pigs was 60.00g B antibody positive pigs, and the positive rate was 93.455.In April 2016, the positive rate of g E antibody positive pigs was #number0# pigs. In October 2016, the positive rate of g E antibody was 92, and the positive rate was 49.20 g B antibody positive pigs. The positive rate of g B antibody was 75.40 in April 2016, and the positive rate of g B antibody in the piglets of 35 days old, 45 days old and 70 days old of pig farm was 70.0050.000.The positive rate of anti-G B antibody of 45 day old piglets was 50.000, which was the appropriate age of first day immunity, and the positive rate was 75.40g B antibody positive rate of 45 days old piglets. The results showed that the infection of pseudorabies in the pig farm was more serious, but the antibody of wild virus showed a downward trend. The serological investigation showed that the prevention and control of pseudorabies was carried out in the pig farm. According to the results of the above test, three different vaccine immunization efficacy comparative tests were carried out in pig pseudorabies negative field and positive field. Sixty healthy piglets of 35 days old were randomly divided into three groups: a vaccine (A vaccine) (domestic SA virus strain B vaccine) (domestic Bartha virus strain C vaccine) (imported Bartha virus strain), one day before the first immunization, and one day before the second immunization. After 28 days of immunization, serum samples were collected to detect the level of antibody against bovis pseudorabies virus E and vaccine GB respectively. The results showed that the positive rate of antibody against pseudorabies was 0, indicating that the pig farm had not been infected by wild virus. The positive rate of antibody against B vaccine was more than 90% on the first day of immunization, and the positive rate was 100 on the 28th day after the second immunization, which was higher than the positive rate of antibody against B vaccine. The positive rate of positive pig farm showed that the positive rate of antibody was 100% on the first day of immunization. The positive rate of positive pig farm showed that the positive rate was 100% on the first day of immunization. There was no significant difference in the positive rate of antibody against g EG B in pigs. The positive rates of antibody against bogus rabies in pigs were 50 ~ 45 and 40g / B respectively one day before the second immunity, and the positive rates of antibody were 50 ~ 6060 ~ 65g / B respectively, and the positive rates were 35404040 / 3535 and 35404040 / 3550 respectively on the 28th day after the second immunization, and the positive rates were 35404040 / 3550 for porcine pseudorabies g / E antibody, respectively. Rabies g B antibody was 750.The vaccine contrast test provided the data basis for the pig farm to select the vaccine according to this field. The infection of pseudorabies wild virus in a large scale pig farm in Heilongjiang showed a downward trend. According to the rule of maternal antibody subtractive, the best age of first immunization was 45 days old. The results of the vaccine test showed that all three vaccines could produce effective antibodies after the second immunization. The immunization effect of domestic SA vaccine and imported Bartha vaccine was better than that of domestic Bartha vaccine, but there was no significant difference between domestic SA vaccine and imported Bartha vaccine.
【学位授予单位】：黑龙江八一农垦大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.28

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