镰刀菌毒素对断奶仔猪肝脏和脾脏损伤的影响

发布时间：2018-02-21 02:50

本文关键词： 镰刀菌毒素仔猪肝脏脾脏损伤　出处：《山东农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：本研究通过对血清肝功能指标、肝脾的抗氧化指标和炎性因子表达量的测定,以及对肝脾组织病理学的观察,系统研究镰刀菌毒素对断奶仔猪肝脏和脾脏损伤的影响以及停饲镰刀菌毒素污染饲粮后仔猪的自然恢复情况。为研究镰刀菌毒素对断奶仔猪肝脏和脾脏损伤的影响,试验选用35日龄、体重为8.45±0.94 kg的健康三元杂交(杜×长×大)雌性仔猪50头,随机分为2组,对照组(Control group)20头,镰刀菌毒素组(Fusarium toxins group)30头,每头猪作为1个重复,对照组饲喂基础饲粮,镰刀菌毒素组饲喂用霉变玉米和霉变玉米蛋白粉替代基础饲粮中50%的玉米和玉米蛋白粉配制而成的镰刀菌毒素污染饲粮(0.90 mg/kg ZEN,1.43 mg/kg DON,5.85 mg/kg FUM),预试期7 d,正试期35 d。正试期结束后每组随机挑选10头仔猪进行屠宰取样。结果表明:(1)与对照组相比,镰刀菌毒素组断奶仔猪血清酶活性显著升高,肝脏蛋白合成能力和抗氧化能力显著降低(P0.05),且血清相应抗氧化指标的变化与肝脏一致;肝脏相对重量显著降低,炎性细胞因子IL-1β和IL-6表达量显著升高(P0.05),且分布发生变化;病理学观察发现肝脏出现肝小叶边缘出现明显的炎性细胞浸润,局部肝细胞出现空泡变性。(2)与对照组相比,镰刀菌毒素组断奶仔猪脾脏的相对重量无明显变化(P0.05),抗氧化能力显著降低(P0.05),且与血清和肝脏中相应指标的变化一致;炎性细胞因子IL-1β和IL-6表达量显著升高(P0.05),且分布发生变化;病理学观察发现脾脏白髓区明显变小,红髓区扩张,且局部出现空泡变性。为研究停饲镰刀菌毒素污染饲粮后断奶仔猪肝脏和脾脏损伤的自然恢复情况,于第一部分屠宰结束后,从镰刀菌毒素组随机挑选10头仔猪为自然恢复组,停止饲喂镰刀菌毒素污染饲粮,改饲基础饲粮,其余仔猪仍在原组,每头猪为1个重复,试验期21 d。试验结束后屠宰所有仔猪并取样。结果表明:与镰刀菌毒素组相比,停饲镰刀菌毒素污染日粮后,断奶仔猪血清酶活性显著降低,肝脏蛋白合成能力得到明显改善(P0.05),但仍未恢复至对照组水平(P0.05);肝脏和脾脏的抗氧化能力均有所提高,且与血清抗氧化指标的变化一致;肝脏和脾脏组织炎性细胞因子IL-1β和IL-6表达量均显著降低(P0.05),且与对照组差异均不显著(P0.05)。以上研究表明,镰刀菌毒素(0.90 mg/kg ZEN,1.43 mg/kg DON,5.85 mg/kg FUM)可引起断奶仔猪血清肝功能指标的改变、肝脏和脾脏抗氧化能力的降低以及组织炎性细胞因子表达的异常,从而造成肝脏和脾脏的损伤;停饲镰刀菌毒素污染饲粮后,断奶仔猪的肝脏和脾脏损伤有明显恢复,但并不能完全恢复至正常水平。
[Abstract]:In this study, the indexes of serum liver function, the antioxidant index of liver and spleen and the expression of inflammatory factors were measured, and the histopathology of liver and spleen were observed. The effects of Fusarium toxin on liver and spleen injury in weaning piglets and the natural recovery of piglets after stopping feeding with Fusarium toxin contaminated diet were studied systematically in order to study the effects of Fusarium toxin on liver and spleen injury in weaned piglets. A total of 50 female piglets were randomly divided into two groups: control group)20 head and Fusarium toxins group)30 head in control group and Fusarium toxins group)30 head in Fusarium toxins group)30 group. The control group was fed with basic diet. The Fusarium toxin group was fed with moldy corn and mildew corn protein powder instead of 50% corn and corn protein powder from basal diet. The Fusarium toxin contaminated diet was 0.90 mg/kg Zen 1.43 mg/kg DON 5.85 mg/kg FUMN. The pretest period was 7 days, and the trial period was 35 days. At the end of the trial period, 10 piglets were randomly selected for slaughter sampling. The serum enzyme activity of weaned piglets in Fusarium toxin group was significantly increased, the protein synthesis and antioxidation ability of liver were significantly decreased (P 0.05), the changes of serum antioxidant indexes were consistent with those of liver, and the relative weight of liver was significantly decreased. The expression of inflammatory cytokines IL-1 尾 and IL-6 was significantly increased and the distribution of P0.05 was changed. Pathological observation showed that there were obvious inflammatory cell infiltration and vacuolar degeneration in the liver at the edge of hepatic lobule, compared with the control group. The relative weight of spleen of weaned piglets in Fusarium toxin group did not change significantly (P 0.05), but the antioxidant capacity decreased significantly, which was consistent with the change of corresponding indexes in serum and liver, and the expression of inflammatory cytokines IL-1 尾 and IL-6 was significantly increased and the distribution was changed. Pathological observation showed that the white medullary area of spleen was obviously smaller, the red pulp area was dilated, and vacuolar degeneration occurred locally. In order to study the natural recovery of liver and spleen injury in weaning piglets after stopping feeding with Fusarium toxin contaminated diet, After the first part was slaughtered, 10 piglets were randomly selected from the Fusarium toxin group as natural recovery group. After 21 days, all piglets were slaughtered and sampled. The results showed that the serum enzyme activity of weaned piglets was significantly lower than that of Fusarium toxin group. The protein synthesis ability of liver was improved obviously, but it did not return to the control level, and the antioxidant ability of liver and spleen was improved, which was consistent with the change of serum antioxidant index. The expression of inflammatory cytokines IL-1 尾 and IL-6 in liver and spleen were significantly decreased, and there was no significant difference between the two groups. The above studies showed that the liver function of weaned piglets could be changed by Fusarium toxin 0.90 mg/kg Zen 1.43 mg/kg DON 5.85 mg/kg FUM. The decrease of anti-oxidation ability of liver and spleen and the abnormal expression of inflammatory cytokines in liver and spleen resulted in liver and spleen injury. But it is not completely back to normal.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S859.8

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