不同饲养方式对苏尼特羊背最长肌肌纤维类型组成的影响
发布时间:2018-02-21 23:44
本文关键词: 苏尼特羊 饲养方式 肌纤维类型 MyHC基因 出处:《食品科学》2017年19期 论文类型:期刊论文
【摘要】:以12月龄不同饲养条件下(放牧和圈养)苏尼特羊背最长肌作为实验材料,用ATP酶染色法和实时定量聚合酶链式反应技术研究不同饲养方式下肌纤维类型组成。结果表明:放牧苏尼特羊背最长肌Ⅰ型和ⅡB型肌纤维直径和横截面积均显著小于圈养(P0.05);同时,放牧苏尼特羊背最长肌Ⅰ型和ⅡA型肌纤维数量比例和面积比例、MyHCⅠ和MyHCⅡa基因的mRNA表达量均显著大于圈养(P0.05),ⅡB型肌纤维数量比例和MyHCⅡb型基因mRNA表达量则呈现相反趋势。
[Abstract]:The longissimus dorsi muscle of Sunita sheep was used as experimental material under different feeding conditions of 12 months old (grazing and captivity). The types of muscle fibers under different feeding conditions were studied by ATP staining and real-time quantitative polymerase chain reaction. The results showed that the diameter and cross-sectional area of longissimus dorsi type 鈪,
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