地方蛋鸡配套系禽白血病净化的初步研究

发布时间：2018-02-24 21:29

本文关键词： 禽白血病蛋鸡配套系净化　出处：《中国家禽》2017年05期 　论文类型：期刊论文

【摘要】：基于地方蛋鸡配套系禽白血病流行病学调查结果,采用ELISA试剂盒检测抗原抗体与病毒分离相结合的方法对地方蛋鸡配套系进行了禽白血病净化研究。结果显示,经过一个世代的净化更替,父系净化组鸡群p27抗原阳性率由第一世代的23.3%、21.7%下降到7.1%、6.8%,ALV-J抗体阳性率由第一世代的21.7%、23.3%下降到8.2%、8.6%,病毒分离阳性率由第一世代的7.5%、6.7%下降到3.2%、3.1%;而母系净化组p27抗原阳性率由第一世代的40.8%、38.3%下降到9.6%、10.1%,ALV-A/B抗体阳性率由第一世代的7.5%、6.7%下降到3.7%、3.2%,鸡群病毒分离阳性率由9.2%、9.2%下降到2.6%、4.0%;净化组配套系鸡群所产的商品代鸡群ALV阳性率也明显低于对照组,父系净化1组(FJ1)和母系净化1组(MJ1)杂交后所产的"净商品1"鸡群胎粪p27抗原阳性率为4.3%,ALV-A/B抗体阳性率为2.4%,ALV-J抗体阳性率为3.0%,病毒分离阳性率为3.0%。结果表明该净化程序效果明显,适于进一步推广应用。
[Abstract]:Based on the epidemiological investigation results of avian leukemia in local layers, the purification of avian leukemia was studied by using ELISA kit to detect antigens and antibodies combined with virus isolation. After a generation of purification, The positive rate of p27 antigen in flocks of paternal purification group decreased from 23.37% in the first generation to 7.1% in the first generation and the positive rate of ALV-J antibody decreased from 21.723% in the first generation to 8.2% in the first generation. The positive rate of virus isolation decreased from 7.56.7% in the first generation to 3.2% in the maternal purification group, while the positive rate of p27 antigen in the maternal purification group decreased from 7.56.7% to 3.23.1.The positive rate of p27 antigen in maternal purification group decreased from 7.56.7% in the first generation to 8.2% in the first generation. The positive rate of ALV-A / R antibody decreased from 40.8% in the first generation to 10.1% in the first generation and decreased from 7.5% to 3.2% in the first generation, and the positive rate of virus isolation decreased from 9.22% to 2.64.0.The positive rate of ALV in the commercial substitute group of the purifying group was also significantly lower than that in the control group. The positive rate of the meconium p27 antigen of the "net commodity 1" chicken herd after the hybridization of maternal purification group 1 (FJ1) and maternal purification group 1 (the positive rate of meconium p27 antigen was 4.3%, the positive rate of ALV-A / B antibody was 2.4% and the positive rate of virus isolation was 3.0%). The results showed that the purification procedure had obvious effect on meconium p27 antigen. It is suitable for further popularization and application.
【作者单位】：江苏省家禽科学研究所;
【基金】：江苏省农业科技自主创新资金(CX(13)3070)
【分类号】：S858.31

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