棘球绦虫检测及棘球蚴病综合防控措施的示范与应用

发布时间：2018-02-27 11:45

本文关键词： 包虫病流行病学防控技术示范应用效果评价　出处：《新疆农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：本研究首先开展了终末宿主感染细粒棘球绦虫检测方法比较研究,结果表明:人工感染细粒棘球绦虫实验犬含卵粪样用饱和蔗糖(1.28 g/mL)漂浮虫卵最佳时段为2 h,可检出虫卵量与其他时段差异显著(P0.05);人工感染实验犬经化学药物驱虫前、后,粪样经粪抗原检测,OD值差异极显著(P0.01);对12288份流行病学调查中收集的家/牧犬粪样进行粪抗原检测,阳性样品991份,感染率为8.1%(991/12288),对991份阳性粪样进行虫卵漂浮法复检,检查出54份粪样中含虫卵;对312只家/牧犬通过氢溴酸槟榔碱下泻法检查,其中,262只犬下泻,检查出27只犬感染细粒棘球绦虫成虫;对262份下泻犬粪样进行粪抗原检测,阳性29份,用虫卵漂浮法检查,仅14份粪样检出虫卵。以裕民县做为包虫病综合技术集成与示范项目点开展中间宿主和终末宿主的基线调查,建立专门的防控工作小组,明确各小组的分工,并与各乡(镇)场签订防疫合同,在农区和牧区分别由县疫控中心和县兽医站负责辖区内的“犬犬投药、月月驱虫”、家/牧犬登记管理、宣传和监督等具体防控的落实与实施;不定期抽样检查各辖区犬服药情况,落实每月所有犬都服用驱虫药物,投药密度达80%以上,包虫病的防控宣传密度达85%以上。以细粒棘球绦虫犬粪抗原抗体夹心ELISA检测方法检测到第1年的感染率做为犬细粒棘球绦虫感染率的基线调查。5年采取8222份犬粪样进行效果考核,犬细粒棘球绦虫感染率从第1年的21.53%(110/511)明显下降到0.88%(24/2737),差异极显著(P0.01);绵羊包虫病感染率从第1年的55.08%(271/492)降至第5年的4.93%(31/629),发放包虫病宣传材料94000余册,包虫病的知晓率为76.48%(803/1050),预防包虫病的知晓率为64.38%(676/1050)。裕民县包虫病的防控工作取得显著地效果。
[Abstract]:In this study, a comparative study on the detection of Echinococcus granulosus infection with terminal host was carried out. The results showed that the best time for floating eggs of Echinococcus granulosus infected dogs with saturated sucrose at 1.28 g / mL was 2 h, and the number of eggs was significantly different from that of other periods (P0.05), and the artificial infected dogs were treated with chemical drugs before and after deworming. The OD value of fecal samples detected by fecal antigen was significantly different (P0.01), 991 positive samples were collected in epidemiological investigation, the infection rate was 8.1% / 12288%, and 991 fecal samples were reexamined by egg floating method. Fifty-four fecal samples were detected, 312 dogs were examined by arecoline hydrobromide method, among which 262 dogs were found to be infected with Echinococcus granulosus adults, while 262 feces samples were tested for fecal antigen. 29 positive cases were detected by egg floating method, only 14 fecal samples were detected. A baseline investigation of intermediate host and terminal host was carried out in Yumin County as a comprehensive technology integration and demonstration project for hydatid disease, and a special working group for prevention and control was set up. Make clear the division of labor among the groups, and sign epidemic prevention contracts with each township (town) farm. The county epidemic control center and the county veterinary station will be responsible for the "canine feeding, monthly insect repellent" and household / dog registration management in the farm area and the pastoral area, respectively. The implementation and implementation of specific prevention and control, such as publicity and supervision; random sampling and inspection of the situation of the dogs in each district, to ensure that every dog takes insecticide-repellent drugs every month, with a dosing density of more than 80%, The propaganda density of prevention and control of echinococcosis was more than 85%. The infection rate of Echinococcus granulosus in the first year was detected by sandwich ELISA method. The baseline survey of infection rate of Echinococcus granulosus was adopted in 5 years. Sample for effect assessment, The infection rate of Echinococcus granulosus dropped from 21.53 / 51 / 511 in the first year to 0.88% of 2737%, the difference was very significant (P0.01). The infection rate of echinococcosis in sheep dropped from 55.08% to 4.931% 62929% in the fifth year, and more than 94000 copies of publicity materials were distributed. The awareness rate of hydatid disease was 76.48% 803 / 1050 and that of prevention of hydatid disease was 64.38% and 676% 1050% respectively. The prevention and control of hydatid disease in Yumin County achieved remarkable results.
【学位授予单位】：新疆农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S855.9

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