新疆阿勒泰地区牛、羊布鲁氏菌病血清学调查及胶体金检测技术的推广

发布时间：2018-02-28 06:34

本文关键词： 布鲁氏菌病血清学调查新疆阿勒泰地区牛羊　出处：《新疆农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：布鲁氏菌病是由布鲁氏杆菌引起的家畜、人以及多种野生动物共同患病的恶性传染病。布鲁氏菌主要侵害动物的生殖器官,流产现象是最为常见,也是最为典型的临床症状。其中,羊布鲁氏菌病疫情较其余家畜最为严重、其次为牛布鲁氏菌病,并且该病的病原能够在牛、羊等多种家畜间交叉感染,造成动物流产、死胎、睾丸炎、不孕不育,关节肿大等症状。布鲁氏菌病的流行造成畜牧业经济损失严重,人布鲁氏菌病激增,以及公共卫生与肉、乳食品安全隐患。本研究用标准试管凝集试验(SAT)和琥红平板凝集试验(RBPT)两种检测方法对新疆阿勒泰地区,2007年至2014年之间家畜牛和羊进行布鲁氏菌病血清学检测,共检测牛血清429940份,羊血清343798份。结果表明,2007年至2014年阿勒泰地区牛布鲁氏菌病疫情呈逐步上升趋势,2012年牛布鲁氏菌病疫情最为严重,该年阳性检出率高达3.04%(2404/79181)。羊布鲁氏菌病疫情上升趋势较牛布鲁氏菌病疫情上升缓慢,其中2009年和2014年羊布鲁氏菌病阳性率最高,分别为1.17%(209/17869)、1.02%(676/66595)。2014年,在原有两种检测方法的基础上,在阿勒泰地区布尔津县3个乡的牛、羊试点采用RBPT与布鲁氏菌病胶体金检测试纸的检测方法进行布鲁氏菌病的检测。采用胶体金试纸共检测牛血清1539份,牛布鲁氏菌阳性率1.56%(24/1539);羊血清1897份,羊布鲁氏菌病阳性率1.58%(30/2897)。采用RBPT方法检测牛血清12345份,牛布鲁氏菌阳性率1.43%(177/12345),羊血清9926份,阳性率1.65%(164/9926)。结果显示,三个乡中,窝依莫克乡牛、羊布鲁氏菌病的流行情况最为严重。本研究对2007年~2014年间新疆阿勒泰地区的牛、羊布鲁氏菌病进行监测。并尝试使用动物布鲁氏菌病胶体金试纸用于该病的检测,本研究的结果为阿勒泰地区牛、羊布鲁氏菌病的预防和控制提供有力的数据支持。
[Abstract]:Brucellosis is a malignant infectious disease caused by brucellosis in domestic animals, humans and many wild animals. Brucellosis mainly infects the reproductive organs of animals, and abortion is the most common. It is also the most typical clinical symptom. Among them, brucellosis of sheep is more serious than other domestic animals, followed by brucellosis of cattle, and the pathogen of the disease can cross infect cattle, sheep and other domestic animals, resulting in abortion and stillbirth of animals. Orchitis, infertility, swollen joints, etc. The epidemic of brucellosis has caused serious economic losses in animal husbandry, a surge in human brucellosis, and public health and meat. In this study, two methods were used to detect brucellosis in cattle and sheep from 2007 to 2014 in Altay area, Xinjiang, by using standard tube agglutination test (SAT) and succinyl red plate agglutination test (RBPTT). A total of 429940 bovine serum samples and 343798 sheep serum samples were detected. The results showed that the epidemic situation of brucellosis in Altay area from 2007 to 2014 was on the rise, and brucellosis was the most serious in 2012. The positive rate of brucellosis in sheep was as high as 3.04 / 79181. The rising trend of brucellosis in sheep was slower than that in bovine brucellosis. The highest positive rate of brucellosis in sheep was 1.17209 / 178696.076 / 66595on 2009 and 2014, respectively. On 2014, on the basis of the original two methods of detecting brucellosis, RBPT and brucellosis colloidal gold test paper were used to detect brucellosis in cattle and sheep in three townships of Brudin County, Altay. A total of 1 539 bovine serum samples were detected by colloidal gold test paper. The positive rate of brucella was 1.560.The positive rate of brucellosis in sheep was 1.897, and the positive rate of brucellosis was 1.58 / 2897. 12345 samples of bovine serum were detected by RBPT method, the positive rate of brucella was 1.4317% 12345%, the positive rate of sheep serum was 9,926, the positive rate was 1.65164% / 9926%. This study monitored brucellosis in cattle and sheep from 2007 to 2014 in Altay, Xinjiang, and tried to use colloidal gold test paper for the detection of brucellosis. The results of this study provide strong data support for the prevention and control of brucellosis in cattle and sheep in Altay area.
【学位授予单位】：新疆农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S855.12

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