吉林省活禽市场外环境禽流感污染情况调查

发布时间：2018-02-28 08:23

本文关键词： 活禽市场禽流感吉林省　出处：《中国公共卫生》2017年03期 　论文类型：期刊论文

【摘要】：目的目的了解吉林省活禽市场外环境禽流感病毒分布状况。方法于2014年3月1日—10日采集吉林省活禽市场外环境,共抽取370个采样点,每个点抽取50家活禽摊位采集外环境鸡笼涂抹、鸡粪和污水标本,采用实时荧光定量PCR法检测禽流感病毒FluA H5、H9和H7核酸。结果 370个采样点中58个(15.68%)检出阳性标本,各地区标本核酸阳性率有明显差异(P0.05);共采集活禽市场外环境标本1 110份,分别为污水359份,粪便381份,笼具擦拭物370份,其中阳性94份,H9阳性60份,H5阳性30份,H5/H9混合阳性4份,未检测出H7阳性,不同类型标本的阳性检出率无统计学差异。结论吉林省活禽市场环境存在禽流感病毒的污染,主要型别为H9和H5亚型,未发现H7亚型。
[Abstract]:Objective to understand the Jilin province live poultry market environment of avian influenza virus distribution. Methods from March 1, 2014 - 10 day gathering in Jilin province live poultry market environment, collected a total of 370 sampling points, each point selected 50 live poultry stalls collection environment cages smear, chicken manure and sewage samples, by using real-time fluorescence quantitative PCR method detection of avian influenza virus FluA, H5, H9 and H7 nucleic acid. The results of 370 sampling points in 58 (15.68%) were positive, the positive rate of nucleic acid samples have obvious difference regions (P0.05); collected live poultry market environment 1110 samples respectively, 359 sewage, 381 fecal specimens of cage cleaning 370, of which 94 samples were positive, 60 were H9 positive, 30 were positive for H5, H5/H9 mixed positive 4, did not detect H7 positive, the positive rate of different types of samples, no significant difference. Conclusion Jilin province live poultry market environment contamination of avian influenza virus, the main The type of H9 and H5 subtype was not found in H7 subtype.

【作者单位】：吉林省疾病预防控制中心传染病预防控制所;吉林省卫生监测检验中心;
【基金】：吉林省自然科学基金(20140101031JC) 吉林省重点科技攻关项目(20150204005SF)
【分类号】：S855.3

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