蛋氨酸对28～70日龄吉林白鹅生产性能及抗氧化能力的影响

发布时间：2018-02-28 21:12

本文关键词： 吉林白鹅蛋氨酸抗氧化能力生产性能　出处：《吉林农业大学》2017年硕士论文　论文类型：学位论文

【摘要】：本试验采用单因子设计,研究蛋氨酸对28~70日龄吉林白鹅生产性能及抗氧化能力的影响。试验选用28日龄健康的吉林白鹅120只,随机分为4个组,每组3个重复,每个重复10只鹅。试验期42d。试验日粮设4个蛋氨酸水平,基础日粮蛋氨酸水平为0.23%,分别在基础日粮中以0.20%为梯度添加DL-蛋氨酸,4组日粮中总蛋氨酸含量分别为0.23%、0.43%、0.63%、0.83%。测定吉林白鹅生长性能及营养物质代谢率,血清生化指标,血清、肝脏、肠道抗氧化能力。结果表明:蛋氨酸对28~70日龄吉林白鹅平均日增重和饲粮增重比影响显著(P0.05),平均日采食量无显著影响(P0.05)。平均日增重随着蛋氨酸水平增加出现先升高后降低的趋势,饲粮增重比随着蛋氨酸水平增加呈现先降低后升高的趋势,0.63%蛋氨酸水平组平均日增重最大,饲粮增重比最低。蛋氨酸可以提高吉林白鹅营养物质代谢率,蛋氨酸对10周龄吉林白鹅干物质代谢率无显著性影响(P0.05),对粗蛋白,粗脂肪代谢率有显著性影响(P0.05)。0.63%组蛋氨酸水平组干物质,粗蛋白,粗脂肪代谢率最大。蛋氨酸对49和70日龄吉林白鹅血清球蛋白,尿酸含量有显著性影响(P0.05),血清总蛋白和球蛋白含量随蛋氨酸水平增加出现先升高后降低趋势,0.63%蛋氨酸水平组达到最大。血清尿酸和甘油三脂随蛋氨酸水平增加出现先降低后升高趋势,0.63%蛋氨酸水平组最低。蛋氨酸对49日龄鹅血清GSH-Px、SOD活力、MDA、GSH含量有显著性影响(P0.05)。其中0.83%蛋氨酸水平组血清中GSH含量最高,MDA含量最低。70日龄时,0.83%蛋氨酸水平组血清GSH-Px和GSH含量显著性高于0.23%蛋氨酸水平组(P0.05),血清中MDA含量随蛋氨酸水平增加而降低。蛋氨酸可以显著提高49日龄时鹅空肠中T-AOC和CAT活力(P0.05),并显著性降低MDA含量(P0.05)。蛋氨酸对70日龄吉林白鹅空肠T-AOC和MDA含量有显著性影响(P0.05)。日粮蛋氨酸水平为0.83%时,空肠T-AOC最大,CAT活力最强,MDA含量最低。肝脏中T-AOC和GSH-Px活力随蛋氨酸水平增加而显著性增加(P0.05),MDA含量显著性降低(P0.05)。肝脏中SOD和CAT活力随蛋氨酸水平增加出现先升高后降低趋势,0.63%蛋氨酸水平组最高,但0.83%蛋氨酸水平组与0.63%蛋氨酸水平组差异不大。综上,日粮中添加蛋氨酸可提高吉林白鹅生产性能和增强吉林白鹅抗氧化能力。日粮蛋氨酸含量分别为0.63%和0.83%时,28~70日龄吉林白鹅生产性能和抗氧化能力分别最好。
[Abstract]:The effects of methionine on the production performance and antioxidant capacity of Jilin White Goose at 28 ~ 70 days old were studied by a single factor design. 120 healthy Jilin white geese of 28 days old were randomly divided into 4 groups with 3 repeats in each group. There were 10 geese per repeat. The trial period was 42 days. The diet was set at 4 methionine levels. The methionine level of basal diet was 0.23, and the total methionine content in the diet of 4 groups were 0.23 and 0.43 respectively. The growth performance and nutrient metabolism rate, serum biochemical index, serum and liver of Jilin White Goose were measured. The results showed that the effects of methionine on the average daily gain (ADG) and dietary gain ratio of Jilin White Goose at 2870 days were significant (P0.05N), while the average daily intake had no significant effect on P0.05G. The average daily gain (ADG) increased first with the increase of methionine level. The trend of decreasing after high, With the increase of methionine level, the dietary gain ratio decreased first and then increased. The average daily gain of the group with 0.63% methionine level was the largest, and the dietary gain ratio was the lowest. Methionine could improve the nutrient metabolism rate of Jilin White Goose. There was no significant effect of methionine on dry matter metabolism rate of Jilin White Goose at 10 weeks of age, but there was significant effect on crude protein, crude fat metabolism rate and dry matter and crude protein in Met level group. The crude fat metabolism rate was the highest. Methionine was used to treat the serum globulin of Jilin White Goose at the age of 49 and 70 days. The content of uric acid had a significant effect on P0.05, and the contents of serum total protein and globulin increased first with the increase of methionine level, and then decreased to the maximum in the group of 0.63% methionine level, while serum uric acid and triglyceride increased with the increase of methionine level. The activity of SOD in serum of 49-day-old geese and the content of MDA-GSH were significantly affected by methionine. The serum GSH content of the 0.83% methionine group was the highest and the lowest at the age of 70 days. At the age of 70 days, the content of MDA-MDA-GSH in the serum of the 0.83% methionine group was the highest and the lowest. The content of MDA-0.83% was the lowest in the group of 0.83% methionine at the age of 70 days. The levels of serum GSH-Px and GSH in the amino acid group were significantly higher than those in the 0.23% methionine group, and the content of MDA in serum decreased with the increase of methionine level. Methionine could significantly increase the activities of T-AOC and CAT in jejunum of 49-day-old goose (P 0.05). The effect of methionine on the contents of T-AOC and MDA in jejunum of Jilin White Goose at 70 days of age was significant (P 0.05). When the dietary methionine level was 0.83%, the effect of methionine on the content of T-AOC and MDA in jejunum of Jilin White Goose was significant. The activities of T-AOC and GSH-Px in liver increased significantly with the increase of methionine level. The activity of SOD and CAT in liver increased first and then decreased with the increase of methionine level. The highest level of methionine was found in the low trend group with 0.63% methionine level. However, there was no significant difference between the 0.83% methionine level group and the 0.63% methionine level group. The content of methionine in diet was 0.63% and 0.83, respectively, and the production performance and antioxidant capacity of Jilin white goose at 2870 days of age were the best.
【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S835.5

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