环形泰勒虫感染对单核细胞中NOD样受体信号通路相关基因转录的影响

发布时间：2018-03-01 05:30

本文关键词： NLRs real-time PCR 环形泰勒虫转录水平　出处：《中国兽医科学》2017年08期 　论文类型：期刊论文

【摘要】：根据Gen Bank发表的相关序列,分别设计针对牛NOD样受体(NLRs)信号通路10个相关分子NOD1、NOD2、NLRP1、NLRP3、NLRC4、NAIP、RIP2、CASP1、IL-18和IL-1β基因设计Real-time PCR特异性引物,通过优化反应条件后对其特异性、敏感性和重复性进行评价,并分别检测环形泰勒虫感染细胞Ta NM中靶基因的转录水平及其与对照的差异。结果显示,所建立的分别检测10个靶基因的Real-time PCR方法特异均非常,其敏感性均在1~100 copies/L之间;而在F10代Ta NM细胞中,NOD1、NOD2、NLRP1、NLRP3、NLRC4、NAIP、RIP2、CASP1和IL-18基因的转录水平均显著低于对照组外周血单核细胞(P0.01),而IL-1β在F10代Ta NM细胞中的转录水平却显著高于对照组(P0.01)。本研究结果为开展环形泰勒虫对宿主细胞天然免疫机制的研究奠定了基础。
[Abstract]:According to the sequence of Gen published in Bank, were designed according to the bovine NOD like receptor 10 (NLRs) signaling pathway related molecules NOD1, NOD2, NLRP1, NLRP3, NLRC4, NAIP, RIP2, CASP1, IL-18 and IL-1 gene specific primers designed by PCR Real-time, the optimal reaction conditions for the specific evaluation the sensitivity and reproducibility, and to detect the transcription of Ta target genes in NM cells Taylor ring worm infection and its difference with the control. The results showed that the specific Real-time PCR were detected in 10 target genes are very sensitive, both in 1~100 and in F10 copies/L; Ta NM cells NOD1, NOD2, NLRP1, NLRP3, NLRC4, NAIP, RIP2, CASP1, and IL-18 gene transcription level were significantly lower than the control group in peripheral blood mononuclear cells (P0.01), and the transcriptional level of IL-1 beta in F10 generation Ta in NM cells was significantly higher than that of the control group (P0.01) in this study. The results laid the foundation for the study of the natural immune mechanism of the host cell in the ring-shaped Taylor worm.

【作者单位】：中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室;宁夏大学农学院;
【基金】：国家重点基础研究发展计划(973)项目(2015CB150300) 国家自然科学基金项目(31402189、31072130、31372432) 农业科技创新工程(ASTIP)国家肉牛牦牛产业技术体系项目(NBCISCARS-38)
【分类号】：S852.7

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