猪流行性腹泻病毒山西分离株ORF3基因序列分析

发布时间：2018-03-02 19:47

本文选题：猪流行性腹泻　切入点：山西分离株　出处：《华北农学报》2017年04期 　论文类型：期刊论文

【摘要】：为了研究山西地区猪流行性腹泻病毒ORF3基因的遗传变异情况,2014-2015年从山西地区11个地市收集的PEDV阳性病料中扩增到4株PEDV的ORF3基因,并进行序列比对及遗传分析。序列分析结果表明,4株PEDV山西分离毒株的ORF3基因与CV777毒株相比,无核苷酸插入和缺失,有部分核苷酸及氨基酸发生变异;4株PEDV山西分离毒株的ORF3基因之间核苷酸和氨基酸的同源性分别为99.6%~100.0%和98.7%~99.6%,与CV777、疫苗株CV777 truncated和attenuated DR13核苷酸同源性分别为96.6%~97.0%,90.6%,97.6%~98.1%,氨基酸同源性分别为95.1%~96.0%,88.0%,97.1%~98.1%。遗传进化分析结果显示,4株PEDV山西分离毒株与12株2010-2015年我国各地方分离的毒株和2009年分离的CH/JL/09毒株亲缘关系较近;与CV777、LZC、Brl/87和2个疫苗株(CV777truncated和attenuated DR13)亲缘关系较远。
[Abstract]:In order to study the genetic variation of porcine epidemic diarrhea virus (ORF3) gene in Shanxi area, the ORF3 gene of 4 strains of PEDV was amplified from PEDV positive venereal materials collected from 11 cities in Shanxi province in 2014-2015. Sequence alignment and genetic analysis showed that there was no nucleotide insertion and deletion in the ORF3 gene of the four strains of PEDV isolated from Shanxi Province, compared with that of the CV777 strain. Nucleotide and amino acid homology among ORF3 genes of 4 strains of PEDV isolated from Shanxi province were 99.6% and 98.775%, respectively. The nucleotide homology of CV777 truncated and attenuated DR13 were 96.60.97. 0 and 97.698.698.1, respectively, and amino acids were similar to those of CV777 truncated and attenuated DR13. The genetic evolution analysis showed that four PEDV strains isolated from Shanxi Province were closely related to the 12 strains isolated from 2010 to 2015 in China and the CH/JL/09 strains isolated in 2009. The relationship is far from CV777LZC / 87 and two vaccine strains, CV777truncated and attenuated DR13.
【作者单位】：山西省农业科学院畜牧兽医研究所;
【基金】：山西省科技攻关项目(20140311020-2-A;20150311018-3)
【分类号】：S852.651

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