2014年江西地区保育猪CSFV、PRRSV、PCV2和PRV的分子流行病学调查

发布时间：2018-03-04 13:45

本文选题：保育猪　切入点：病原学　出处：《江西农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：目前,疫病是影响江西养猪业的重要因素之一,养猪生产上面临着疫病种类多、病原变异、多病原混合感染等问题。随着日龄的增长,母源抗体水平逐渐下降、断奶应激、疫苗免疫保护未完全形成等因素,保育猪是规模化养猪场中最容易发生疫病(尤其是多病原感染)的猪群。为了解江西地区保育猪的病原的感染及变异情况,本论文从江西部分猪场采集病(死)保育猪病料,采分子生物学方法分析猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型(PCV2)和猪伪狂犬病毒(PRV)四种病毒的感染情况,随机对部分毒株进行测序,分别了解病原的变异情况,为江西地区养猪场进行保育猪的疫病防控提供病原学依据。1.江西地区保育猪的病原感染情况调查2014年1月至2014年12月间,从江西省南昌、宜春、吉安、赣州、抚州、九江和上饶地区的不同规模养猪场采集546份病(死)保育猪病料,对每一份病料均分别进行了猪繁殖与呼吸综合征病毒(PRRSV)、猪瘟病毒(CSFV)、猪圆环病毒2型(PCV2)和猪伪狂犬病毒(PRV)四种病病毒的PCR/RT-PCR检测。结果显示:PRRSV的阳性数为375,占总样品百分率为68.6%;CSFV的阳性数为204,占总样品百分率为37.3%;PCV2的阳性数为275,占总样品百分率为50.3%;PRV的阳性数为70,占总样品百分率为12.8%。2.四种病原的感染类型分析546份病(死)保育病料样品中,单独感染的样品为203份,占总样品数的37.1%;二重混合感染的样品为308份,占总样品数的56.3%;三重感染的样品为30份,占总样品数的5.4%;四重感染的样品为5份,占总样品数的0.9%。调查结果表明:保育猪的病原的多重感染普遍化,PRRSV、CSFV、PCV2和PRV的二重以上混合感染高达62.9%;二重感染中,以“PRRSV+PCV2”或“PRRSV+CSFV”感染为主,分别占二重混合感染的45.1%和31.8%。3.PRRSV、CSFV、PCV2和PRV的变异情况分析(1)12株猪瘟病毒江西流行株的E2基因的核苷酸和氨基酸同源性分别为96.5%-100%和92.4%-100%;江西流行株与CSFV石门株的核苷酸同源性为82.6%-83.3%。通过E2基因的遗传进化树分析中,可将CSFV可分为两大基因群,即基因1群和基因2群,本论文测定的12株猪瘟病毒江西流行株均属于基因2群的2.1b亚群。(2)15株PRRSV毒株ORF5基因的核苷酸和氨基酸同源性为93.7%-99.7%和89.1%-100%。与美洲株(VR2332)的核苷酸和氨基酸同源性为82.4%-87.2%和80.2%-89.1%,与欧洲株(MLV)的核苷酸和氨基酸同源性为79.7%-86.6%和82.1%-86.9%。15株PRRSV江西流行株均属于美洲型。(3)11株PCV2江西流行株的核苷酸和氨基酸同源性为94.2%-99.7%和64.2%-93.2%。遗传进化树分析结果表明:11株江西毒流行株中9株属于PCV2b型,2株属于PCV2a型。(4)9株PRV江西流行株gB基因的核苷酸和氨基酸同源性分别为94.7%-100%和99.5%-99.9%。与BARTHA-2011相比,核苷酸同源性为97.8%-97.9%,与KAPLAN相比,同源性为98.0%-98.2%。遗传进化树分析,9株流行株亲缘关系近。
[Abstract]:At present, epidemic disease is one of the important factors affecting the pig industry in Jiangxi Province. Pig production is faced with many kinds of disease, variation of pathogen, mixed infection of multiple pathogens, and so on. With the increase of age, the level of maternal antibody decreases gradually, and the weaning stress. The vaccine immunity protection is not fully formed, and the care of pigs is the most susceptible to blight disease (especially multi-pathogen infection) in large-scale pig farms. In order to understand the infection and variation of the pathogen of the preserved pigs in Jiangxi area, In this paper, four kinds of viruses, CSFV, PRRSVV, PCV2 and PRV), were collected from some pig farms in Jiangxi province. The infection of CSFV, PRRSVV, PCV2 and PRV) were analyzed by molecular biological methods. Some virus strains were sequenced at random to find out the variation of pathogen, and to provide the etiological basis for the prevention and control of pig diseases in Jiangxi pig farms. 1. The investigation of pathogen infection of preserved pigs in Jiangxi area from January 2014 to December 2014. 546 samples were collected from pig farms of different scales in Nanchang, Yichun, Ji'an, Ganzhou, Fuzhou, Jiujiang and Shangrao regions of Jiangxi Province. PCR/RT-PCR detection of PRRSVV, CSFV, PCV2 and PRV) of porcine reproductive and respiratory syndrome virus (PRRSVV) and porcine pseudorabies virus (PRV2) were carried out respectively. The results showed that the positive number of the virus was 375, accounting for the total sample. The positive rate of CSFV was 204, the percentage of PCV2 was 275, the percentage of PCV2 was 50.3%, the positive number of PRV was 70, and the percentage of total samples was 12.8.2.The infection types of the four pathogens were analyzed in 546 samples. The number of samples infected alone was 203, accounting for 37.1of the total number of samples; the number of samples with double mixed infection was 308, accounting for 56.3% of the total number of samples; the number of samples with triple infection was 30, accounting for 5.4% of the total number of samples; and the number of samples with quadruple infection was 5. The results of the investigation showed that the double infection of PRRSVV, CSFV, PCV2 and PRV was as high as 62.9%, and in the double infection, "PRRSV PCV2" or "PRRSV CSFV" was the main infection. 45.1% and 31.8.3.The variation of PRV and CSFVV2 of PRRSVV virus in Jiangxi Province the nucleotide and amino acid homology of E2 gene of 12 strains of swine fever virus in Jiangxi were 96.5-100% and 92.4-100%, respectively; the nucleotide homology between the prevalent strain of Jiangxi and CSFV strain was 96.5% -100% and 92.4-100%, respectively; the nucleotide homology between the epidemic strain of Jiangxi and the strain of CSFV Shimen strain was 96.5% -100% and 92.4-100%, respectively. The sex is 82.6% -83.3%. In the genetic evolution tree analysis of E2 gene, CSFV can be divided into two groups: group 1 and group 2. In this paper, the nucleotide and amino acid homology of the ORF5 gene of all the 12 prevalent strains of CSFV in Jiangxi were 93.7-99.7% and 89.1-100, respectively, and the homology of nucleotide and amino acid were 82.4- 87.2% and 82.4- 87.2%, respectively, compared with that of the American strain VR2332. The nucleotide and amino acid homology of the ORF5 gene of all the 12 strains of CSFV were 2.1b, 2.1b, 2.1b, 2.1b and 2.1b, respectively. The homology of nucleotide and amino acids with the European strain MLV was 79.7-86.6% and 82.1 -86.9.15 strains of PRRSV Jiangxi were of American type. The nucleotide and amino acid homology of Jiangxi strain were 94.2-99.7% and 64.2-99.7% respectively. The results of genetic evolution tree analysis showed that the genetic evolution tree analysis showed that the genetic evolution tree analysis showed that the nucleotide and amino acid homology was 94.2 -99.7% and 64.2% -29.3%, respectively. The results of genetic phylogenetic tree analysis showed that the nucleotide and amino acid homology was 94.2% -99.7% and 64.2% -29.3% respectively. The nucleotide and amino acid homology of GB gene in 9 strains of Jiangxi virulent strain belonging to PCV2b type, 2 strains belonging to PCV2a type and 9 strains of PRV epidemic strain in Jiangxi province were 94.7- 100% and 99.5- 99.9%, respectively. Compared with BARTHA-2011, the nucleotide and amino acid homology of GB gene in Jiangxi epidemic strain were 94.7% -100% and 99.5- 99.9%, respectively. The nucleotide homology was 97.8- 97.99.Compared with KAPLAN, the homology was 98.0-98.2.The genetic phylogenetic tree analysis showed that 9 epidemic strains were close to each other.
【学位授予单位】：江西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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