利用SNP标记估计西门塔尔牛亲缘关系系数的准确性

发布时间：2018-03-05 10:53

本文选题：西门塔尔牛　切入点：亲缘关系系数　出处：《畜牧兽医学报》2016年02期 　论文类型：期刊论文

【摘要】：本研究旨在利用SNP标记估计西门塔尔牛亲缘关系系数,以期准确确定估计个体间亲缘关系系数所需的SNPs数量。研究以1 059头出生于2008-2012年的西门塔尔牛为试验群体,利用Illumina bovineHD(770k)芯片,根据最小等位基因频率(MAF)区间,分别选择100、500、1 000、1 500、2 000、2 500和3 000个SNPs用于个体间亲缘关系系数的估计。结果显示,随着标记数目的增多,估计的亲缘关系系数准确性逐渐增加。且当SNP标记数目达到2 500时,所估计的亲缘关系系数与利用所有标记估计的个体间亲缘关系系数差异不显著,二者相关系数达到0.89以上。同时,利用不同等位基因频率区间内标记估计的个体间亲缘关系系数差异不显著。由此可以看出,当所选标记数目达到2 500以上时,可以得到较高的亲缘关系系数估计准确性。本研究为基于SNP标记信息估计亲缘关系系数的进一步研究提供了理论基础,同时为西门塔尔牛群体个体间亲缘关系的研究提供依据。
[Abstract]:The purpose of this study was to estimate the genetic relationship coefficient of Simmental cattle by using SNP markers, in order to determine accurately the number of SNPs needed to estimate the genetic relationship coefficient among individuals. The study used 1 059 Simmental cattle born from 2008 to 2012 as the experimental population. Using Illumina bovine HDV 770k chip, according to the minimum allelic frequency (Maffs) interval, 100,500,000,000,000000 and 3,000 SNPs were selected to estimate the genetic relationship coefficient between individuals. The results showed that the number of markers increased with the increase of the number of markers. When the number of SNP markers reached 2 500, there was no significant difference between the estimated genetic coefficients and the estimated genetic coefficients among individuals using all markers. The correlation coefficient was above 0.89. At the same time, there was no significant difference in the coefficient of genetic relationship between individuals estimated by the markers in different allelic frequency intervals. It can be seen that when the number of markers selected is more than 2,500, This study provides a theoretical basis for the further study of estimating the genetic coefficients based on SNP markers and provides a basis for the study of the genetic relationship among individuals in Simmental cattle population.
【作者单位】：吉林农业大学动物科技学院;中国农业科学院北京畜牧兽医研究所;
【基金】：国家自然科学基金(31402039) 北京市自然科学基金(6154032) 中国农业科学院基本科研业务费(2014ywf-yb-4) 农业部物种资源保护(畜禽)项目(F557) 中国农业科学院科技创新工程经费(cxgc-ias-03) 科技支撑计划(2011BAD28B04) 863项目(2013AA102505-4) 中国农业科学院院本级增量业务费(2013ZL031)
【分类号】：S823

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