2014年～2016年某大型规模化猪场猪繁殖与呼吸综合征病毒的遗传演化分析

发布时间：2018-03-05 23:08

本文选题：猪繁殖与呼吸综合征病毒　切入点：NSP　出处：《中国预防兽医学报》2017年01期 　论文类型：期刊论文

【摘要】：为研究近年来猪繁殖与呼吸综合征病毒(PRRSV)在规模化猪场的变异情况,本研究对华北某规模化猪场2014年~2016年采集的117份疑似PRRS的病料进行PCR检测,结果阳性病料57份,并对阳性病料中PRRSV的NSP2基因和ORF5基因测序分析。结果显示,43份阳性病料中的PRRSV为NSP2缺失1+29个氨基酸,与HP-PRRSV的缺失相同,其NSP2基因区与HP-PRRSV Hu N4株的同源性为93.6%~99.6%;而其余的14份阳性样品中的病毒NSP2则为缺失111+1+19个氨基酸,与PRRSV NADC30株的缺失相同,NSP2基因区同源性为92.3%~92.9%,ORF5基因同源性为93.8%~94.8%。此外,在2016年检测到的4份阳性病料,基于NSP2基因构建的系统进化树显示其属于HP-PRRSV亚群,但基于ORF5基因构建的系统进化树结果显示,这些病毒则与国内近年来在香港、台湾以及华南地区流行的低致病力PRRSV GM2株(谱系3)同源性最高,核苷酸同源性为96.7%~97.0%。以上结果表明2014年以来同一猪场内的PRRSV变异呈多样性,并且出现了两类新的PRRSV变异株。
[Abstract]:In order to study the variation of porcine reproductive and respiratory syndrome virus (PRRSVV) in large scale pig farm in recent years, 117 samples of suspected PRRS collected from a large scale pig farm in North China were detected by PCR from 2014 to 2016. The NSP2 gene and ORF5 gene of PRRSV were sequenced and analyzed. The results showed that the PRRSV of 43 samples was NSP2 deletion of 129 amino acids, which was the same as that of HP-PRRSV. The homology of the NSP2 gene region with HP-PRRSV Hu N4 strain was 93.66.The virus NSP2 in the remaining 14 positive samples was 111,19 amino acids deletion, and the homology of the same nucleotide sequence of NSP2 gene with that of the PRRSV NADC30 strain was 93.894.88.In addition, the homology of the NSP2 gene region was 93.894.85.In addition, the homology of the NSP2 gene region with that of the PRRSV NADC30 strain was 93.894.894. In 2016, the phylogenetic tree constructed based on NSP2 gene showed that it belonged to HP-PRRSV subgroup, but the phylogenetic tree based on ORF5 gene showed that these viruses were similar to those in Hong Kong in recent years. The PRRSV GM2 strains with low pathogenicity (lineage 3) have the highest homology and the nucleotide homology is 96.77.00 in Taiwan and South China. The above results indicate that the PRRSV mutation in the same pig farm has been diverse since 2014, and two new PRRSV variants have appeared.
【作者单位】：中国农业科学院哈尔滨兽医研究所;南阳师范学院;中国农业科学院上海兽医研究所;
【基金】：国家自然科学基金(31270045);国家自然科学基金(31502097)
【分类号】：S852.651

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