高谷物日粮促进山羊瘤胃上皮单羧酸转运蛋白1及钠钾ATP酶mRNA的表达

发布时间：2018-03-06 13:59

  本文选题：高谷物日粮　切入点：瘤胃上皮　出处：《草业学报》2017年02期 　论文类型：期刊论文

【摘要】：本试验旨在研究高谷物日粮对山羊瘤胃上皮形态结构及单羧酸转运蛋白(monocarboxylate transporter,MCT)和钠钾ATP酶mRNA表达的影响。将10头装有永久性瘤胃瘘管的健康阉割公山羊随机分为饲喂全粗料日粮的对照组(Hay,0%谷物,n=5)和饲喂高谷物日粮的处理组(HG,65%谷物,n=5),试验期为7周。试验开始后,于每周晨饲后的0、2、3、4、6、8和12h连续采集瘤胃液监测瘤胃pH值的变化,收集其中第0、3、6和12h的瘤胃液待测挥发性脂肪酸(volatile fatty acid,VFA)浓度。试验的第50天,屠宰采集瘤胃上皮用于形态学及基因定量分析。研究结果显示:与全粗料组山羊相比,高谷物组山羊瘤胃pH值、乙酸浓度及乙丙比都显著下降(P0.001),而瘤胃丙酸浓度、丁酸浓度及其他VFA浓度都显著升高(P0.001);高谷物日粮组的瘤胃乳头长度显著高于对照组(P=0.001),瘤胃乳头宽度显著低于对照组(P0.001),但是两组间的瘤胃乳头表面积并无显著差异;透射电镜结果显示,长期饲喂高谷物日粮导致瘤胃上皮细胞线粒体发生降解;实时定量PCR结果表明,与对照组相比,高谷物日粮显著升高了MCT1(P0.001)和钠钾ATP酶(P=0.001)的mRNA表达量,显著降低了MCT4的mRNA表达量(P=0.041),但对MCT2的表达没有显著影响(P=0.305);进一步分析这些基因的mRNA表达量与pH值和VFA浓度之间的相关性,结果显示,MCT1和钠钾ATP酶的mRNA表达量与瘤胃pH值和乙酸浓度呈显著负相关,与总VFA、丙酸、丁酸的含量呈显著正相关,而MCT4的mRNA表达量与pH值呈显著正相关,与总VFA、丙酸、丁酸的含量呈显著负相关。以上结果提示:高精料引起的瘤胃pH值下降和VFA的变化可能与瘤胃上皮MCT和钠钾ATP酶表达量的变化相关。研究结果对深入认识高谷物饲喂引发的瘤胃功能紊乱具有重要意义。
[Abstract]:The purpose of this study was to study the effects of high grain diet on the morphological structure of rumen epithelium and the expression of monocarboxylate transporterase mRNA and monocarboxylate transporterase mRNA in goats. Ten healthy castrated male goats with permanent rumen fistula were randomly divided into feeding. The control group (Hayan 0%) and the treatment group fed high grain diet (HGN 65%) were used for 7 weeks, the trial period was 7 weeks. The rumen fluid was collected continuously for monitoring the rumen pH value after feeding every morning, and the concentration of volatile volatile fatty acid (volatile fatty acid) volatile fatty acido (VFA) in rumen fluid was collected at the 0th and 12h after feeding continuously. The concentration of volatile fatty acid volatile fatty acid (volatile fatty acid) in the rumen fluid was determined on the 50th day of the experiment, and the concentration of volatile fatty acid (VFA) in the rumen fluid was determined at 12 h and 0 h after feeding. The results showed that the rumen pH value, acetic acid concentration and ethylene-propyl ratio in the high grain group decreased significantly compared with the whole crude diet group, while the rumen propionic acid concentration decreased significantly in the slaughtering and collecting rumen epithelium, while the rumen propionic acid concentration in the high grain group was significantly lower than that in the whole crude diet group. Butyric acid concentration and other VFA concentrations were significantly higher than that of control group (P 0.001), the rumen papilla length of high grain diet group was significantly higher than that of control group, and the rumen papilla width was significantly lower than that of control group, but there was no significant difference in rumen papilla surface area between the two groups. The results of transmission electron microscope showed that the degradation of mitochondria in rumen epithelial cells was induced by long-term feeding of high grain diet, the results of real-time quantitative PCR showed that the high grain diet significantly increased the mRNA expression of MCT1 + P0.001) and sodium and potassium ATP enzyme P0. 001) compared with the control group. The mRNA expression of MCT4 was significantly decreased, but the expression of MCT2 was not significantly affected. The correlation between the mRNA expression of these genes and pH value and VFA concentration was further analyzed. The results showed that the mRNA expression of MCT1 and sodium and potassium ATP was negatively correlated with rumen pH and acetic acid concentration, and positively correlated with the contents of total VFA, propionic acid and butyric acid, while the mRNA expression of MCT4 was positively correlated with pH value, and with total VFA-propionic acid. The results suggested that the decrease of rumen pH and the change of VFA might be related to the changes of MCT and ATP expression in rumen epithelium. Rumen dysfunction caused by feeding is of great significance.
【作者单位】： 江苏省消化道营养与动物健康重点实验室南京农业大学消化道微生物研究室;
【基金】：国家自然科学基金(31172228)资助
【分类号】：S827.5

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