松针多糖对小鼠腹腔巨噬细胞免疫调节作用的研究

发布时间：2018-03-07 12:04

本文选题：松针多糖　切入点：巨噬细胞　出处：《动物营养学报》2017年02期 　论文类型：期刊论文

【摘要】：本试验旨在研究松针多糖对正常状态及脂多糖(LPS)刺激状态下小鼠腹腔巨噬细胞的免疫调节作用。试验采用不同浓度的松针多糖作用于正常的和经LPS刺激的小鼠腹腔巨噬细胞,设空白对照组(加入100μL RPMI-1640培养基)、阳性对照组(加入100μL终浓度为5μg/m L的LPS)、松针多糖组(分别加入100μL 25、50、100、200μg/m L的松针多糖)和LPS+松针多糖组(加入与松针多糖组相同浓度的松针多糖和终浓度为5μg/m L的LPS,液体终体积为200μL)。噻唑蓝(MTT)比色法检测细胞活力,中性红吞噬试验检测巨噬细胞吞噬能力,Griess法检测一氧化氮(NO)的分泌量,酶联免疫吸附测定(ELISA)法检测巨噬细胞培养上清液中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)的分泌量。结果表明:1)对空白对照组相比,各松针多糖组巨噬细胞相对增殖率均显著或极显著提高(P0.05或P0.01),50、100和200μg/m L松针多糖组巨噬细胞中性红吞噬率显著或极显著提高(P0.05或P0.01),各LPS+松针多糖组巨噬细胞中性红吞噬率均极显著提高(P0.01),200μg/m L松针多糖组巨噬细胞NO分泌量极显著提高(P0.01),50、100和200μg/m L松针多糖组巨噬细胞TNF-α和IL-1β分泌量显著或极显著提高(P0.05或P0.01),50、100和200μg/m L松针多糖组巨噬细胞IL-10分泌量极显著降低(P0.01)。2)与阳性对照组相比,各松针多糖组巨噬细胞相对增殖率均极显著降低(P0.01),100和200μg/m L LPS+松针多糖组巨噬细胞中性红吞噬率极显著升高(P0.01),50、100和200μg/m L LPS+松针多糖组巨噬细胞NO分泌量极显著提高(P0.01),各LPS+松针多糖组巨噬细胞TNF-α分泌量显著或极显著提高(P0.05或P0.01),50、100和200μg/m L LPS+松针多糖组巨噬细胞IL-1β分泌量显著或极显著提高(P0.05或P0.01),100和200μg/m L LPS+松针多糖组巨噬细胞IL-10分泌量极显著降低(P0.01)。由此可见,松针多糖通过发挥其促炎作用调节巨噬细胞的免疫功能,进而增强机体抗疾病的能力。
[Abstract]:This experiment was conducted to study the pine polysaccharides on normal and lipopolysaccharide (LPS) stimulation of immune function of peritoneal macrophages in mice under the condition of test. With different concentrations of Polysaccharides from pine needles on normal and stimulated by LPS in mouse peritoneal macrophages, blank control group (adding 100 L RPMI-1640 medium), positive control group (adding 100 L final concentration of 5 g/m L LPS), pine polysaccharide group (100 L 25,50100200 were added to g/m L pine polysaccharide) and LPS+ group (adding polysaccharides from pine needles and pine needle polysaccharides were the same concentration of Polysaccharides from pine needles and a final concentration of 5 g/m L LPS finally, the liquid volume is 200 L). Methyl thiazolyl tetrazolium (MTT) cell vitality assay, neutral red phagocytosis test to detect macrophage phagocytosis, detection of nitric oxide Griess (NO) secretion, enzyme linked immunosorbent assay (ELISA) method for the detection of macrophage cell culture supernatant in white blood cells Cell interleukin -1 beta (IL-1 beta), tumor necrosis factor alpha (TNF- alpha), interleukin -10 (IL-10) secretion. The results show that: 1) compared to the control group, the pine polysaccharide group macrophage relative proliferation rate were significantly increased (P0.05 or P0.01). 50100 and 200 g/m L pine polysaccharide group macrophage neutral red phagocytosis rate significantly higher (P0.05 or P0.01), the LPS+ pine polysaccharide group neutral red phagocytosis rate of macrophages increased significantly (P0.01), 200 g/m L pine polysaccharide group macrophage NO secretion increased significantly (P0.01). 50100 and 200 g/m L pine polysaccharide group macrophage TNF- alpha and IL-1 beta secretion significantly improve (P0.05 or P0.01), 50100 and 200 g/m L pine polysaccharide group significantly decreased the secretion of macrophage IL-10 (P0.01).2) compared with the positive control group, each group of macrophages relative polysaccharides from pine needles the proliferation rate was extremely significant Reduce (P0.01), 100 and 200 g/m L LPS+ pine polysaccharide group neutral red phagocytosis rate of macrophages increased significantly (P0.01), 50100 and 200 g/m L LPS+ pine polysaccharide group macrophage NO secretion increased significantly (P0.01), the LPS+ pine polysaccharide group of macrophage TNF- alpha secretion significantly increased significantly (P0.05 or P0.01), 50100 and 200 g/m L LPS+ pine polysaccharide group IL-1 beta macrophage secretion significantly higher (P0.05 or P0.01), 100 and 200 g/m L LPS+ pine polysaccharide group macrophage IL-10 secretion decreased significantly (P0.01). Thus, polysaccharides from pine needles through its proinflammatory effects regulate macrophage immune function, thereby enhancing the body's ability to resist disease.

【作者单位】：江西省天然产物与功能食品重点实验室江西农业大学食品科学与工程学院;江西工业贸易职业技术学院粮食工程系;
【基金】：国家科技支撑计划(2013BAD10B04-3) 江西省科技厅农业处重点项目(2016BBF60086)
【分类号】：S816.7

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