药物影响斗鸡攻击行为及其调控基因的研究

发布时间：2018-03-08 05:22

本文选题：吐鲁番斗鸡　切入点：攻击行为　出处：《石河子大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的与意义:通过“药物影响斗鸡攻击行为及其调控基因的研究”分析吐鲁番斗鸡攻击行为与其血浆中皮质醇(CORT)等激素、5-羟色胺转运体(5-HTT)等基因表达量的关系,探明影响吐鲁番斗鸡攻击行为的影响因素。不仅有助于吐鲁番斗鸡攻击行为性状的选育以及其遗传资源的保护和开发利用,而且对其他动物(包括人类)攻击行为与内分泌系统和5-HTT等基因的相关性的深入研究在理论与实践上都具有重要意义。内容与方法:以吐鲁番斗公鸡为试验材料,采用攻击行为评分法、酶联免疫法和QPCR等方法,分组研究不同剂量人用抗暴力性精神分裂症的利培酮、阿立哌唑、曲唑酮、舍曲林4种药物对吐鲁番斗鸡攻击行为表型、CORT、MT、T、TSH的含量以及5-HTT、COMT、TPH1、DTNBP1基因的表达量的影响。结果与分析:(1)结果显示口服利培酮等四种药物后吐鲁番斗鸡的攻击行为均呈现不同程度的减弱。对利培酮、阿立哌唑、、舍曲林(A4、B4组)均是第1天与第14天差异显著(P0.05),舍曲林(C4组)第1天与第11、14天差异显著(P0.05);其他组间、组内均差异不显著(P0.05)。(2)不同剂量利培酮和阿立哌唑组吐鲁番斗鸡血浆中CORT含量组内均呈下降趋势,且随着药物剂量的增加和用药时间的增长趋于显著水平,曲唑酮各组呈上升趋势但差异不显著(P0.05)。利培酮、曲唑酮组MT含量增加,阿立哌唑、舍曲林组组内MT含量下降,且差异均不显著(P0.05)。四种药物对T和TSH含量的影响均不显著(P0.05)。(3)利培酮和阿立哌唑使5-HTT基因的表达呈下调趋势,5-HTT:利培酮组A1、B1组第1、2、5与11、14天5-HTT的表达量差异显著(P0.05);C1组第1、2与8、11、14天差异显著(P0.05);阿立哌唑组:A2组第2与14天差异显著(P0.05),B2组第1、2、5与8、11、14天差异显著(P0.05),C2组第1与5、8、11、14天差异显著(P0.05)。TPH1:利培酮组A1组第1与11差异极显著(P0.01),第1、2、5、8与14差异显著(P0.05),第2、5、8与11差异显著(P0.05);B1组第1、2、5、8与11、14天差异显著(P0.05);C1组1与5、8、11、14差异显著(P0.05),第2与5、8、11差异显著(P0.05),第2与14天差异极显著(P0.01),阿立哌唑组:A2组第1、2与14差异显著(P0.05),B2组第1与14差异显著(P0.05),C2组第2与14天差异显著(P0.05)。舍曲林、曲唑酮组5-HTT表达量呈上升趋势,对其他三种基因无明显影响,差异不显著(P0.05)。结论:四种人用治疗精神分裂症的药物均可不同程度减少吐鲁番斗鸡的攻击行为,其中以利培酮的减弱作用较为显著。随着吐鲁番斗鸡攻击行为的减弱,CORT含量减少,利培酮、阿立哌唑组5-HTT基因含量降低,TPH1基因含量增加,说明CORT、5-HTT基因与斗鸡攻击行为呈正相关、TPH1基因与斗鸡攻击行为呈负相关,因此,可将CORT的含量与5-HTT基因、TPH1基因的表达量作为吐鲁番斗鸡攻击行为选育的一个参考指标,为吐鲁番斗鸡攻击行为的激素与分子选育奠定基础。
[Abstract]:Aim and significance: to analyze the relationship between the aggressive behavior of Turpan cockfighting and the expression of 5-hydroxytryptamine transporter (5-HTT) in plasma by "study on the effect of drugs on aggressive behavior and its regulatory gene". The factors that influence the aggressive behavior of Turpan cocks are proved, which are not only helpful to the breeding of aggressive behavior traits of Turpan cocks, but also to the protection and utilization of their genetic resources. Furthermore, it is of great significance in theory and practice to study the relationship between aggressive behavior of other animals (including humans) and endocrine system and 5-HTT genes. Risperidone, aripiprazole, trazodone in different doses of human anti-violent schizophrenia were studied by aggressive behavior score, enzyme-linked immunosorbent assay (Elisa) and QPCR. The effects of sertraline on the content of TSH and the expression of DTNBP1 gene of 5-HTTTh1 TPH1 gene in Turpan cocks after oral risperidone were analyzed. The results showed that the aggressive behavior of Turpan cocks after taking risperidone and other four drugs was obvious. Varying degrees of abatement. For risperidone, Aripiprazole, sertraline A _ 4 / B _ 4) were significantly different on day 1 and day 14 (P 0.05), sertraline C 4) on day 1 and day 1114 (P 0.05). There was no significant difference in plasma CORT content between different doses of risperidone and aripiprazole in Turfan cocks, and it tended to be significant with the increase of drug dosage and time. The content of MT in trazodone group increased, while that in aripiprazole group and sertraline group decreased. No significant difference was found in the expression of 5-HTT gene between risperidone and aripiprazole. The expression of 5-HTT gene was down-regulated by risperidone and aripiprazole. There were significant differences on day 1 and day 1 between group 1 and group 2 on day 1, P 0.05, and on day 1, 2, and on day 1, 2, and on day 1, 1, 1, 1, 1, 1 and 14, respectively. The difference between group 1 and group 1 was significant (P 0.05), and that between group 1 and group 1 was significantly higher than that of group B 2 on day 14 (P 0.05). There were significant differences between group A 1 and group A 1 and group 1 (P 0.011), and the difference between group 1 and group 1 was very significant (P 0.011), and that between group 1 and group 1 was significantly higher than that of group B 2 on day 1, and day 1 with day 1, day 1, with day 1, day 1 and day 14 respectively. There were significant differences in P0.05, P0.05, P0.05 and 11 in group 1, P 0.05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 01, P 0. 01, P 0. 01, P 0. 01, P 0. 01, P 0. 01, P 0. 01, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 01, P 0. 01, P 0. 01, P 0. 01, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, P 0. 05, respectively. There were significant differences between 1 and 14 groups on the 2nd and 14th days of P0.05C _ 2 group (P 0.05), sertraline, sertraline (P 0.05), and sertraline (P 0.05). The expression of 5-HTT in trazodone group showed an upward trend, but had no obvious effect on the other three genes, but the difference was not significant (P 0.05). Conclusion: four kinds of drugs for treatment of schizophrenia can reduce the aggressive behavior of Turpan chickens in different degrees. With the decrease of attack behavior of Turpan cocks, the content of risperidone and aripiprazole reduced the content of 5-HTT gene, and the content of TPH1 gene increased with the decrease of attack behavior of Turpan cocks, and the decrease of 5-HTT gene content in risperidone and aripiprazole groups. These results suggested that the expression of CORT and the expression of 5-HTT gene could be used as a reference index for the selection of aggressive behavior of Turpan cockfighting. To lay a foundation for hormone and molecular breeding of attack behavior of Turpan cock.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S831

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