白术中黄酮的提取及其抑菌和抗氧化活性的研究

发布时间：2018-03-08 18:48

  本文选题：白术　切入点：黄酮　出处：《黑龙江畜牧兽医》2017年12期 　论文类型：期刊论文

【摘要】：为了研究白术中黄酮的抑菌与抗氧化活性,为白术资源的深度开发利用提供依据,试验以总黄酮提取率为评价指标,采用正交试验优化了白术中黄酮的超声提取工艺,并采用最小抑菌浓度法和清除二苯代苦味肼基自由基(DPPH·)法测定了其抑菌和抗氧化活性。结果表明:白术黄酮超声提取的最佳工艺条件为,乙醇浓度为60%,料液比(g/m L)为1∶40,在50℃下超声提取35 min,在该条件下黄酮的提取率为1.38%;白术黄酮对金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌均有较强的抑菌活性,而对酵母菌和产青霉菌则没有抑制作用;白术黄酮及维生素C对DPPH·的半清除率(IC50)分别为17.26μg/m L、12.68μg/m L,说明白术黄酮具有较强的抗氧化活性。
[Abstract]:In order to study the bacteriostatic and antioxidation activities of flavonoids in Atractylodes macrocephala and provide the basis for the deep exploitation and utilization of Atractylodes macrocephala resources, the extraction rate of total flavonoids was taken as the evaluation index. The bacteriostatic and antioxidation activities of Atractylodes macrocephala flavonoids were determined by the method of minimal inhibitory concentration and scavenging diphenyl bitter hydrazinyl radical DPPH 路. The results showed that the optimum conditions for ultrasonic extraction of flavone from Atractylodes macrocephala were: 1. The ethanol concentration was 60%, the ratio of material to liquid was 1: 40, the ultrasonic extraction time was 35 min at 50 鈩，

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