谷氨酰胺干预断奶仔猪肠道黏膜受损中免疫能力的作用

发布时间：2018-03-08 22:34

本文选题：谷氨酰胺　切入点：免疫　出处：《畜牧兽医学报》2017年04期 　论文类型：期刊论文

【摘要】：本试验旨在研究日粮中添加谷氨酰胺(Gln)对大肠杆菌型脂多糖(LPS)诱导肠道黏膜损伤后断奶仔猪免疫性能的影响。选取28日龄健康断奶仔猪24头,随机分为3组,每组8个重复。其中,空白对照组和LPS组饲喂常规基础日粮,Gln组饲喂添加1%谷氨酰胺的基础日粮。试验期30d,于试验期第22、25、28、30天对LPS组和Gln组腹腔注射LPS(按体重以100μg·kg-1给予),空白对照组腹腔注射等体积的无菌生理盐水。第30天对仔猪进行前腔静脉采血并屠宰采取所需样品,检测血清中免疫球蛋白、相关炎症因子浓度及各肠段中这些炎症因子基因的表达丰度。与空白对照组相比,LPS组和Gln组血清IgM水平显著提高40.00%和62.86%(P0.05);LPS组血清IL-8含量显著提高7.11%(P0.05),而Gln组则无明显变化。不论在LPS攻毒前还是攻毒后,日粮添加Gln均可显著提高仔猪血清IL-17含量(P0.05)。与空白对照组相比,LPS组和Gln组十二指肠中IL-8mRNA表达水平显著降低了35.06%和44.16%(P0.05)。对于IL-12βmRNA的表达水平,在空肠和回肠中Gln组中的表达水平较LPS组高,但在十二指肠中有所降低(P0.05)。在十二指肠与空肠中,IL-17αmRNA的表达丰度在三组间存在差异(P0.05),特别是在十二指肠和回肠中,Gln组的表达丰度达到最高;LPS组和Gln组空肠中IL-17αmRNA相对表达量显著降低58.16%和56.07%(P0.05)。结果提示,日粮中添加1%的谷氨酰胺能够在一定程度上改善LPS诱导肠道损伤后断奶仔猪的免疫力,减轻应激反应造成的机体损伤。
[Abstract]:The aim of this study was to study the effect of glutamine glutamine (Gln) on immune performance of weaned piglets after intestinal mucosal injury induced by lipopolysaccharide (LPS). 24 healthy piglets of 28 days of age were randomly divided into 3 groups. 8 repeats per group. Of which, The blank control group and the LPS group were fed the basic diet supplemented with 1% glutamine. On the 30th day of the trial period, the LPS group and the Gln group were injected intraperitoneally with LPS (100 渭 g 路kg-1) on the 30th day of the trial period, and the blank control group was intraperitoneally injected with 100 渭 g 路kg-1. The piglets were collected blood from the anterior vena cava on the 30th day and slaughtered to take the required samples. Detection of immunoglobulin in serum, Compared with the control group and Gln group, the serum IgM level in the LPS group and the Gln group was significantly increased by 40.00% and 62.86% respectively, and the serum IL-8 level in the LPS group was significantly higher than that in the Gln group, but not in the Gln group. Change. Whether before or after LPS attack, Dietary supplementation of Gln significantly increased the serum IL-17 content of piglets. Compared with the control group, the IL-8mRNA expression level in duodenum of lipopolysaccharide group and Gln group decreased significantly by 35.06% and 44.16. The expression level of IL-12 尾 mRNA was significantly decreased. The expression level of Gln in jejunum and ileum was higher than that in LPS group. However, the expression of IL-17 伪 mRNA in duodenum and jejunum was different among the three groups, especially in duodenum and ileum. The expression abundance of IL-17 伪 mRNA in jejunum of LPS group and Gln group was the highest. The expression levels were significantly decreased by 58.16% and 56.07, respectively. Adding 1% glutamine to the diet could improve the immunity of weaned piglets after intestinal injury induced by LPS to a certain extent and alleviate the damage caused by stress reaction.
【作者单位】：湖南农业大学动物科学技术学院;中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室湖南省畜禽健康养殖工程技术中心农业部中南动物营养与饲料科学观测实验站;中国科学院大学;湘潭大学;
【基金】：国家973课题(2013CB127301) 中国科学院百人计划项目国家自然科学基金面上项目(31472107)
【分类号】：S828.5

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