陆川猪MC1R和KIT基因型鉴定

发布时间：2018-03-09 20:00

本文选题：陆川猪　切入点：毛色　出处：《中国畜牧兽医》2017年03期 　论文类型：期刊论文

【摘要】：为了检测陆川猪种群的纯度,本研究以4个华南地方猪种(莆田黑猪、粤东黑猪、大花白猪、巴马香猪)和3个外来猪种(杜洛克猪、大白猪、长白猪)作为对照,利用PCR测序法对陆川猪群体56个样本的MC1R和KIT基因进行基因型鉴定。测序结果表明,大白猪和长白猪KIT基因内含子17的第1个碱基发生GA剪接突变,而陆川猪和4个华南地方猪种,以及杜洛克猪中KIT基因型一样,均为野生型的GG基因型;MC1R基因编码区分析表明,以海南野猪MC1R基因型作为参考序列,陆川猪和4个华南地方猪种在MC1R基因编码区存在95ValMet和102LeuPro两个错义突变;MC1R基因非编码区分析表明,与大白猪、长白猪和杜洛克猪相比,陆川猪和4个华南地方猪种MC1R基因的5′UTR分别存在5~6个SNPs,3′UTR存在1个A碱基的缺失。另外,试验对一窝毛色异常的陆川猪仔猪及其亲本的KIT和MC1R基因进行测序分析,结果表明,仔猪及其亲本的KIT基因型均为GG野生型,仔猪和母本的MC1R基因均存在ED1和Ep两种不同的基因型,父本的MC1R基因型为ED1,因Ep对应于皮特兰猪的斑块表型,所以初步判定母本渗入了外来猪种的血统。本研究通过检测陆川猪等8个猪种的毛色相关基因KIT和MC1R基因的基因型,证实了中外猪种间在毛色遗传上的分子差异,为今后深入研究猪毛色遗传的分子调控机理提供参考。
[Abstract]:In order to test the purity of Luchuan pig population, four South China local pig breeds (Putian black pig, eastern Guangdong black pig, big flower white pig, Bama Xiang pig) and three foreign pig breeds (Duroc, big white, Landrace) were used as control. The genotypes of MC1R and KIT genes in 56 samples of Luchuan pig population were identified by PCR sequencing. The results showed that GA splicing mutation occurred in the first base of intron 17 of KIT gene in large white pig and Landrace pig. However, the KIT genotypes of Luchuan pig, four local South China pigs and Duroc pigs were all wild type GG genotypes and MC-1R gene coding region analysis showed that the MC1R genotype of Hainan wild boar was taken as the reference sequence. The analysis of 95 ValMet and 102 LeuPro missense mutations of MC1R gene in the coding region of MC1R gene in Luchuan pig and four local swine breeds in South China showed that compared with large white pig, Landrace pig and Duroc pig, there were two missense mutants in Luchuan pig, Landrace pig and Duroc pig. In Luchuan pig and 4 local swine breeds in South China, 5 ~ 6 SNPs 3 MC1R genes were found in 5 ~ 6 MC1R genes. In addition, the KIT and MC1R genes of a litter of piglets with abnormal coat color were sequenced and analyzed. The KIT genotypes of piglets and their parents were all GG wild type. The MC1R gene of both piglets and mothers had two different genotypes, ED1 and EP. The MC1R genotype of male parent was ED1, because EP corresponded to the patch phenotype of Pieterland pig. Therefore, it was preliminarily determined that the female parent infiltrated into the lineage of foreign pig breeds. By detecting the genotypes of KIT and MC1R genes related to coat color in eight pig breeds, including Luchuan pigs, this study confirmed the molecular differences in coat color inheritance between Chinese and foreign pig breeds. It provides a reference for further study on molecular regulation mechanism of pig coat color inheritance.
【作者单位】：中山大学生命科学学院有害生物控制与资源利用国家重点实验室;广东壹号食品股份有限公司;
【基金】：科技基础性工作专项(2014FY120800) 广东省省级科技计划(2014B020202001) 广东省扬帆计划(2014YT02H042) 广东省自然科学基金(2016A030313310) 国家转基因生物新品种培育重大专项(2016ZX08006003-006)
【分类号】：S828

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