鸭源NDV激活鸡和鸭先天性免疫应答的比较研究

发布时间：2018-03-09 22:00

  本文选题：新城疫病毒(NDV)　切入点：鸡　出处：《农业生物技术学报》2017年02期 　论文类型：期刊论文

【摘要】：鸡(Gallus gallus)和鸭(Anas platyrhynchos)是新城疫病毒(Newcastle disease virus,NDV)的主要宿主,但对于NDV感染的反应差异显著,先天性免疫应答在其中起重要作用。为了比较鸭源NDV激活鸡和鸭先天性免疫应答的不同,阐明先天性免疫应答在宿主致病性差异产生中的作用,以SD03(鸭源NDV)对鸡和鸭的致病性比较研究为基础,利用荧光定量PCR对不同时间点外周血中模式识别受体(pattern recognition receptors,PRRs)、细胞因子、抗病毒蛋白和主要组织相容性复合体分子(major histocompatibility complex,MHC)m RNA的表达量进行检测和比较。结果表明,SD03在鸡脾脏中的复制效率高于鸭;免疫基因中Toll样受体7(toll-like receptor 7,TLR-7)、白介素1β(interleukin 1β,IL-1β)、IL-2、IL-6、IL-8、干扰素β(interferonβ,IFN-β)、MHCⅠ和MHCⅡ在鸡和鸭外周血中的表达模式不同且表达量变化趋势差异显著;除IL-2外,所有检测基因在鸡外周血中的表达量明显高于鸭。研究结果在机体水平上展示了NDV诱导鸡和鸭先天性免疫反应的不同,并分析了其在致病性差异产生中的作用,为全面理解NDV对不同禽类的致病机制提供了数据支持。
[Abstract]:Gallus gallus) and Anas platyrhynchos) are the main hosts of Newcastle disease virus (NDV), but there are significant differences in the response to NDV infection, and innate immune response plays an important role. To elucidate the role of congenital immune response in the pathogenicity difference of host, based on the comparative study of pathogenicity between chicken and duck by SD03 (duck NDV), fluorescence quantitative PCR was used to detect the pattern recognition receptor pattern recognition receptor (PRRsN), cytokines in peripheral blood at different time points. The expression of MHCm RNA of major histocompatibility complex was detected and compared with that of major histocompatibility complex. The results showed that the replication efficiency of MHCM RNA in chicken spleen was higher than that in duck. The expression patterns of Toll like receptor 7toll-like receptor 7tLR-7, interleukin-1 尾 interleukin-1 尾 -interleukin-1 尾 -interleukin-1 尾 -interleukin-1 尾 / IL-2IL-6 / IL-8, interferon 尾 -interferon 尾 -IFN- 尾 -IFN- 尾 and MHC 鈪，

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