山羊CMKLR1基因的克

发布时间：2018-03-13 11:04

  本文选题：山羊　切入点：CMKLR基因　出处：《中国畜牧兽医》2017年06期 　论文类型：期刊论文

【摘要】：试验旨在克隆山羊CMKLR1基因序列并进行生物信息学分析,研究其表达与肌内脂肪(IMF)沉积的相关性。采集山羊皮下脂肪组织,应用RT-PCR克隆山羊CMKLR1基因序列;以GAPDH(GenBank登录号:AJ431207.1)为内参基因,实时荧光定量PCR检测其在山羊不同组织中的表达量及在肌肉组织中的时序表达谱(以肝脏表达量为基准);测定肌肉组织中的IMF含量,分析其与CMKLR1mRNA表达量的相关性。结果显示,克隆获得了山羊CMKLR1基因,1 089bp的CDS区,GenBank登录号:KT165374。实时荧光定量PCR分析显示,CMKLR1基因在24月龄山羊心脏、肝脏、脾脏、肺脏、肾脏、脂肪、背最长肌、股二头肌和臂三头肌中均存在不同程度的表达,其中在肺脏中表达量显著高于其他组织(P0.05);CMKLR1基因在1~3和24月龄的山羊背最长肌、臂三头肌和股二头肌中表达趋势相同,均是在股二头肌中表达量最高,而8~10月龄则是在臂三头肌中表达量最高。山羊IMF含量以24月龄背最长肌含量最高。山羊背最长肌、股二头肌和臂三头肌中CMKLR1mRNA表达与IMF含量相关性不显著(P0.05)。CMKLR1基因不参与山羊IMF沉积作用,试验结果为进一步研究CMKLR1基因奠定理论基础。
[Abstract]:The aim of the experiment was to clone goat CMKLR1 gene sequence and analyze its expression by bioinformatics, and to study the correlation between the expression of goat CMKLR1 gene and intramuscular fat (IMF) deposition. The goat subcutaneous adipose tissue was collected and goat CMKLR1 gene sequence was cloned by RT-PCR. Using GAPDH(GenBank accession number AJ431207.1 as internal reference gene, real-time fluorescence quantitative PCR was used to detect its expression in different tissues of goat and its temporal expression profile in muscle tissue. The content of IMF in muscle tissue was measured. The results showed that the CMKLR1 gene of goat was cloned and cloned into GenBank accession number: 1: KT165374.The real-time fluorescence quantitative PCR analysis showed that the CMKLR1 gene was expressed in heart, liver, spleen, lung, kidney, fat of goat at 24 months of age. The expression of CMKLR1 gene in the longissimus dorsi muscle, biceps femoris muscle and triceps arm muscle was significantly higher than that in other tissues, and the expression trend of CMKLR1 gene in the triceps arm and biceps femoris muscle was the same. The highest expression was found in biceps femoris muscle, and the highest expression was found in triceps arm muscle at the age of 8 to 10 months. The IMF content of goat was the highest in the 24 month old longissimus dorsi muscle, and the highest in the goat dorsal longissimus muscle at the age of 8 to 10 months. There was no significant correlation between CMKLR1mRNA expression and IMF content in biceps femoris and triceps arm muscle. The results of the experiment laid a theoretical foundation for further study of CMKLR1 gene.
【作者单位】： 青藏高原动物遗传资源保护与利用四川省重点实验室;贵州省播州区泮水镇农业服务中心;西南民族大学生命科学与技术学院;
【基金】：国家自然科学基金(31672395、31601921) 四川省“十三五”畜禽育种攻关项目(2016NZ0099-36) 四川省科技创新产业链示范工程重大项目(2014NZ0003)
【分类号】：S827
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本文编号：1606102