荨麻对鼠小肠和子宫运动影响的研究

发布时间：2018-03-13 11:17

本文选题：麻叶荨麻　切入点：狭叶荨麻　出处：《新疆农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：新疆荨麻资源丰富,尤其以麻叶荨麻(Urtica Cannabina,UC)和狭叶荨麻(Urtica Angustifolia,UA)分布最广。为了探究荨麻对平滑肌运动的影响,本研究比较了UC和UA对鼠子宫和小肠运动的影响,并运用受体或离子通道阻断策略初步探讨其可能机制,以期为荨麻用于治疗胃肠或子宫运动异常疾病提供参考。首先,用生物信号处理系统检测了浓度递增的UC和UA醇提液对未孕大鼠离体子宫平滑肌运动的影响及机制。结果显示,UC浓度递增到4.0%时较用药前显著增加子宫收缩频率(P0.05),递增到4.0%~8.0%时较用药前显著降低收缩幅度,但对子宫运动张力影响不明显;加入0.5%UA后子宫的收缩运动受到抑制,但浓度递增到2.0%以后,子宫收缩张力增加,频率加快,当浓度递增到8.0%时子宫出现僵直并且张力逐渐下降,直到完全抑制。UC(2.0%)和M-受体阻断剂阿托品(6.2×10-6 mol/L)联合用药,较用药前使子宫收缩频率显著加快,收缩张力增加(P0.05),但较UC单独用药显著减低收缩张力(P0.05)。其次,探究了浓度递增的UC和UA醇提液对禁食大鼠离体小肠平滑肌运动的影响及可能作用的机制。结果显示,当UC浓度递增到2.0%时能引起小肠收缩频率、收缩张力和收缩幅度增加,在UC浓度递增到4.0%~8.0%时,收缩频率和收缩幅度较用药前显著降低(P0.05)。加入0.5%UA小肠运动表现短暂性僵直趋势,之后随着浓度递增表现为收缩张力和幅度增加,但当浓度递增到2.0%~4.0%时开始出现抑制作用。钙离子通道阻滞剂维拉帕米(2×10-7 mol/L)和UC(2.0%)联合用药较用药前以及UC单独用药显著抑制小肠的收缩频率、收缩幅度和收缩张力(P0.05)。将UC分别同酚妥拉明、雷尼替丁、苯海拉明和吲哚美辛联合用药,对小肠运动影响同UC单独用药没有差异(P0.05)。最后,测定了10.0%、2.0%和0.4%UC对禁食小鼠在体胃排空率和肠推进率的影响。结果显示,各浓度UC、生理盐水空白对照和氨甲酰甲胆碱阳性对照均使小鼠胃排空率达95%以上,并且各组之间差异不明显(P0.05)。在对肠推进率方面,各浓度UC和氨甲酰甲胆碱较生理盐水组显著增加小肠推进率(P0.05),阿托品单独用药较同0.4%UC联合用药显著减小小肠推进率(P0.05),且这两组均较生理盐水组减小小肠推进率(P0.05)。本研究结果表明,UC剂量依赖性促进大鼠子宫和肠平滑肌运动,而UA在不同浓度时对离体子宫和肠平滑肌运动表现出兴奋或抑制的双向效应。UC对胃肠和子宫运动的兴奋作用可能同激动M-受体和钙离子通道有关,荨麻有望用于治疗胃肠或子宫动力不足相关疾病。
[Abstract]:Urtica Cannabina (UCC) and Urtica Angustifolia UAA (Urtica Angustifolia) are the most abundant resources in Urtica angustifolia. In order to investigate the effect of Urtica Angustifolia (UAA) on smooth muscle movement, the effects of UC and UA on the movement of uterus and small intestine in mice were compared. In order to provide reference for the treatment of gastrointestinal or uterine dysmotility, the receptor or ion channel blocking strategy was used to explore the possible mechanism of urticaria. The effects of increasing concentration of UC and UA on the movement of uterine smooth muscle in vitro of non-pregnant rats were investigated by using biological signal processing system. The results showed that the frequency of uterine contraction was significantly increased when the concentration of UCU increased to 4.0. The amplitude of contraction was significantly decreased when P0. 05% was increased to 4. 0 ~ 8. 0. The contractile movement of uterus was inhibited after adding 0.5 UA, but after increasing to 2.0%, the uterine contraction tension increased and the frequency increased. When the concentration increased to 8.0, the uterus became stiff and the tension gradually decreased. Up to complete inhibition (UCN 2.0) and M- receptor blocker atropine (6.2 脳 10 -6 mol / L), the uterine contraction frequency was significantly increased and the contraction tension was increased (P 0.05), but the contraction tension was significantly decreased than that of UC alone. The effects of increasing UC and UA concentrations on the movement of isolated small intestinal smooth muscle of fasting rats and the possible mechanism were investigated. The results showed that the increase of UC concentration to 2.0 could induce the contraction frequency, contraction tension and contraction amplitude of small intestine. When the concentration of UC increased to 4.0 ~ 8.0, the contractile frequency and amplitude were significantly lower than those before the treatment. The intestinal motion showed a transient rigidity trend after adding 0.5UA, and then the contractile tension and amplitude increased with the increase of the concentration. But the inhibition began to appear when the concentration increased to 2.0-4.0. The calcium channel blocker verapamil (2 脳 10-7 mol / L) and UC2.0) significantly inhibited the contraction frequency of small intestine compared with those before treatment and UC alone. Contractile amplitude and contraction tension (P0.05). There was no difference in the effect of UCU with Phenoto#internal_person0#, ranitidine, phenhydramine and indomethacin on the small intestine motility compared with UC alone (P0.05). The effects of 2.0% and 0.4UC on gastric emptying rate and intestinal propulsive rate in fasting mice were determined. The results showed that the gastric emptying rate of mice was more than 95% in each concentration of UC, normal saline blank control and carbamylcholine positive control. And there was no significant difference among the groups (P 0.05). Compared with normal saline group, all concentrations of UC and carbamylcholine significantly increased the small intestinal propulsive rate (P0.05), and atropine alone significantly decreased the intestinal propulsive rate (P0.05N) compared with the same 0.4 UC group, and both groups decreased the intestinal propulsive rate (P0.05N) compared with the normal saline group (P 0.05). The results showed that UCU promoted the smooth muscle movement of uterus and intestine in a dose-dependent manner. At different concentrations, UA showed a bidirectional effect of stimulation or inhibition on the smooth muscle movement of isolated uterus and intestine. The stimulation effect of UC on gastrointestinal and uterine motility may be related to the activation of M- receptor and calcium channel. Urticaria is expected to be used to treat gastrointestinal or uterine dysmotility related diseases.
【学位授予单位】：新疆农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7

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