牛至油对小鼠灌胃给药的急性毒性试验

发布时间：2018-03-16 13:25

本文选题：牛至油　切入点：急性毒性　出处：《动物医学进展》2017年05期 　论文类型：期刊论文

【摘要】：牛至油作为一种新型动物保健药物,具有促进生长、抗菌、杀菌、增强机体免疫力等作用,然而其毒性大小尚未见报道。采用Bliss法设计,对昆明小鼠单次灌胃牛至油,测定其对小鼠半数致死量(LD_(50))及其95%可信限,观察7d内小鼠的毒性反应和死亡情况,对小鼠进行剖检和组织病理学检查。结果显示,牛至油单次灌胃小鼠出现不同程度的毒性反应,包括自发活动减少、闭眼、俯卧、震颤、毛发直立、强制性-阵挛性抽搐、角弓反张等,LD_(50)为688.33mg/kg,95%可信限为584.33mg/kg~806.34mg/kg。死亡小鼠均毛发直立、个体消瘦;剖检均可见肝、肾肿大;组织病理学检查可见高剂量组肝、肾组织受损明显,肝细胞索排列紊乱,肝细胞疏松,肝血窦扩张;肾小球严重肿胀,肾小囊腔狭窄,肾小管上皮细胞有不同程度肿胀、坏死。综上所述,牛至油对小鼠单次灌胃具有一定毒性,属于低毒物质。
[Abstract]:As a new animal health drug, oregano oil has the functions of promoting growth, antibacterial, sterilizing and enhancing immunity. However, the toxicity of oregano oil has not been reported yet. The Bliss method was used to design a single dose of oregano oil in Kunming mice. The 50% lethal dose (LD50) and its confidence limit of 95% were measured, the toxicity and death of mice were observed within 7 days, and the histopathological examination was performed on the mice. The results showed that, The toxic effects of single oral administration of oregano oil in mice included decreased spontaneous activity, eye closure, prone position, tremor, erect hair, mandatory clonic convulsion, angle arch reversion, etc.) the confidence limit of 95% confidence limit was 688.33 mg / kg 95%, 584.33 mg / kg 路kg 806.34 mg / kg 路kg 路kg ~ (-1). In the histopathological examination, the liver was obviously damaged, the hepatic cell cord was disordered, the hepatocyte was loose, the hepatic blood sinusoidal dilated, the glomerular swelling was serious, the renal capsule was narrow, the liver tissue was damaged obviously in the histopathological examination. The tubule epithelial cells were swollen and necrotic in varying degrees. In conclusion, oregano oil has certain toxicity on single gastric perfusion of mice, which is a low toxic substance.
【作者单位】：西北师范大学生命科学学院;
【基金】：西北师范大学青年教师科研能力提升计划项目(NWNU-LKQN-13-8) 国家自然科学基金项目(81660589)
【分类号】：S859.7

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