黑羽番鸭PRL、PRLR基因序列分析及表达规律研究

发布时间：2018-03-16 23:18

本文选题：黑羽番鸭　切入点：RACE扩增　出处：《中国家禽》2017年19期 　论文类型：期刊论文

【摘要】：催乳素(Prolactin,PRL)由动物垂体前叶分泌,与其受体(Prolactin receptor,PRLR)相结合才能作用于靶细胞。为了解PRL与PRLR基因在黑羽番鸭不同繁殖阶段各组织内的时空表达规律,利用RACE扩增方法获得了PRL与PRLR基因的CDS区序列,同时应用荧光定量与免疫组化相结合的方法,对产蛋前期、产蛋期、就巢期的黑羽番鸭垂体、卵巢、子宫中PRL与PRLR基因的表达量进行了比较分析。结果显示:PRL与PRLR基因序列在多种禽类中高度同源;PRL基因在垂体、卵巢、子宫中m RNA的表达具有明显的空间特异性,PRL基因在垂体中表达量最高,极显著高于卵巢和子宫中的表达量(P0.01),这种趋势贯穿产蛋前期、产蛋期和就巢期。PRLR基因在不同时期各组织内m RNA的表达具有明显的时空特异性。PRLR基因的表达量在垂体、卵巢中的表达量普遍较低,子宫中的表达量最高。PRLR基因在不同时期垂体和卵巢中的表达量没有明显的差异(P0.05),而子宫中的表达量在就巢期显著高于产蛋前期与产蛋期(P0.05)。
[Abstract]:Prolactin prolactinus prolactinus (PRL) was secreted from the anterior pituitary gland of animals and combined with its receptor Prolactin receptor (PRLR). In order to understand the temporal and spatial expression of PRL and PRLR genes in different tissues of black muscovy duck at different reproductive stages, The sequence of CDS region of PRL and PRLR gene was obtained by RACE amplification. Meanwhile, the pituitary gland and ovary of black feathered Muscovy duck during egg laying, egg laying and nesting were analyzed by fluorescence quantitative and immunohistochemical method. The expression of PRL gene and PRLR gene in uterus were compared and analyzed. The results showed that the sequence of PRL gene and PRLR gene were highly homologous in pituitary and ovary. The expression of m RNA in uterus was the highest in pituitary gland, which was significantly higher than that in ovary and uterus. The expression of m RNA in different tissues during egg laying and nesting stage had obvious temporal and spatial specificity. The expression of m RNA gene was generally low in the pituitary gland, and was generally low in the ovary. There was no significant difference in the expression of PRLR gene between pituitary and ovary at different stages, but the expression of PRLR gene in uterus was significantly higher than that in prelaying stage and egg laying stage (P0.05).
【作者单位】：江苏农牧科技职业学院;国家级水禽基因库(江苏);
【基金】：泰州市科技支撑项目(TN201503)
【分类号】：S834.2

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