外源性IL-6与TNF-α对乳腺炎奶牛乳汁中性粒细胞凋亡的影响

发布时间：2018-03-18 21:31

本文选题：奶牛　切入点：乳腺炎　出处：《黑龙江畜牧兽医》2016年17期 　论文类型：期刊论文

【摘要】：为了明确外源性白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)对奶牛乳腺炎乳汁中性粒细胞凋亡的影响,试验分离乳腺炎奶牛乳汁的中性粒细胞并进行体外培养,以不同浓度的外源性IL-6(0.05 ng/m L、0.1 ng/m L、0.2 ng/m L、0.5 ng/m L)与TNF-α(2 ng/m L、5 ng/m L、10 ng/m L、20 ng/m L)以及IL-6和TNF-α联合液(1∶100,1∶50,100∶1,50∶1,1∶1)对其进行作用,在不同时间点(6,12,24,48,72小时)收集细胞,应用流式细胞仪检测细胞凋亡的情况。结果表明:外源性IL-6对乳腺炎奶牛乳汁中性粒细胞凋亡产生抑制作用;TNF-α在6~48 h范围内对乳腺炎奶牛乳汁中性粒细胞凋亡产生促进作用;IL-6与TNF-α按不同比例混合后共同作用于体外培养的奶牛乳汁中性粒细胞后,IL-6占比多时以抑制细胞凋亡为主,TNF-α占比多时以促进细胞凋亡为主。说明外源性IL-6和TNF-α能影响乳腺炎奶牛乳汁中性粒细胞的凋亡,能为奶牛乳腺炎的预防和治疗提供试验依据。
[Abstract]:In order to investigate the effects of exogenous interleukin-6 (IL-6) and tumor necrosis factor- 伪 (TNF- 伪) on the apoptosis of bovine mastitis milk neutrophils, neutrophils from mastitis milk were isolated and cultured in vitro. The cells were collected at different concentrations of exogenous IL-6(0.05 ng/m L0. 1 ng/m Ln0. 2 ng/m L0. 5 ng/m L), TNF- 伪 2 ng/m L5 ng/m Ln10 ng/m L0. 20 ng/m L) and IL-6 and TNF- 伪 combined solution 1: 100: 1: 50: 50: 1: 1: 1: 1: 1: 1: 1). The cells were collected at different time points. Apoptosis was detected by flow cytometry. The results showed that exogenous IL-6 inhibited the apoptosis of milk neutrophils in mastitis cows. TNF- 伪 produced apoptosis in milk neutrophils of mastitis cows within 648 hours. After mixed with TNF- 伪 in different proportion, IL-6 and TNF- 伪 can inhibit apoptosis of milk neutrophils and promote apoptosis in vitro. It is suggested that exogenous effect of TNF- 伪 is the most important factor in promoting apoptosis of dairy cow milk neutrophilic granulocytes in vitro, and TNF- 伪 plays an important role in promoting cell apoptosis when the proportion of IL-6 and TNF- 伪 is high. IL-6 and TNF- 伪 can affect the apoptosis of milk neutrophils in mastitis cows. It can provide experimental basis for the prevention and treatment of cow mastitis.
【作者单位】：内蒙古农业大学兽医学院农业部动物疾病临床诊疗技术重点实验室;
【基金】：内蒙古自治区自然基金项目(2012MS0419)
【分类号】：S858.23

【相似文献】