松辽黑猪NR6A1和VRTN基因多态性与肋骨数的关联分析

发布时间：2018-03-18 22:32

本文选题：松辽黑猪　切入点：NR6A1基因　出处：《吉林农业大学》2015年硕士论文　论文类型：学位论文

【摘要】：在生产过程中发现松辽黑猪群体中的不同个体之间存在肋骨数量的差异。结合松辽黑猪的选育历史,为了解析松辽黑猪群体中肋骨数的差异,本研究以松辽黑猪为试验对象群体,选用杜长大三元杂交猪为试验对照群体,分别采集了173头松辽黑猪和283头杜长大三元杂交猪的背最长肌组织样本为试验材料,提取基因组DNA,并且按采样顺序记录了肋骨数表型值。分别选取与脊椎数量显著相关的NR6A1和VRTN为候选基因,运用PCR-RFLP和基因分型技术,筛选候选基因的多态位点,并进一步与肋骨对数表型值进行相关分析,以期为松辽黑猪在品种内选育和提高多肋品系提供比较理想的分子标记和理论依据。本研究取得结果如下:NR6A1基因的相关研究区域在杜长大三元杂交群体中不存在基因多态性,但在松辽黑猪群体中存在基因多态性,在目的片段184bp处存在AG突变;VRTN基因的相关研究区域在杜长大三元杂交群体和松辽黑猪群中存在基因多态性,在目的片段112bp处存在一个大约289bp的插入突变;验证了NR6A1基因目的片段的Nci I酶切位点在杜长大三元杂交群体内存在选择清除效应,NR6A1基因目的片段的NciI酶切位点在松辽黑猪群体内存在AA、AG、GG三个基因型,且该酶切位点在松辽黑猪群体内处于Hardy-Weinberg平衡状态,遗传多态性处于中度多态;VRTN基因目的片段的ins/ins、ins/-和-/-三种基因型在杜长大三元杂交群体和松辽黑猪群体内均存在,该插入突变位点在杜长大三元杂交群体和松辽黑猪群体均处于Hardy-Weinberg平衡状态,遗传多态性处于中度多态;NR6A1基因的184AG位点松辽黑猪个体的肋骨数表型变异现象不存在关联性,VRTN基因112bp位点的插入突变与杜长大三元杂交猪和松辽黑猪的肋骨数表型变异现象存在一定的关联性,因此该插入突变位点很有可能成为松辽黑猪在品种内选育和提高多肋品系的理想分子标记。
[Abstract]:In the production process has found differences between different individual rib number of Songliao Black pig group. Combined with the breeding history of Songliao Black pig, the difference in order to rib in Songliao Black pig population number analysis, this study in Songliao Black Pig as the experimental object groups, with three yuan of Duroc crossbred pigs as experimental control group, respectively. Collect 173 Songliao Black Pigs and 283 head three yuan Duroc Hybrid Pigs longissimus muscle tissue samples as test materials, the genomic DNA was extracted, and according to the sampling sequence records were selected. The phenotypic value of rib was significantly associated with vertebral number of NR6A1 and VRTN as a candidate gene by PCR-RFLP and gene typing technique screening of candidate genes, polymorphism, and further with the pair of ribs of phenotypic values were analyzed in order to provide an ideal molecular marker and the rationale for the Songliao Black Pig breeding and raising in varieties of multi rib lines On the basis of this study. The results are as follows: the related research region of the NR6A1 gene in Duroc gene polymorphism does not exist three hybrid populations, but the presence of genetic polymorphisms in Songliao Black pig population, the existence of AG mutation in 184bp fragment; related research region of the VRTN gene in the gene polymorphism of three Duroc crossbred groups and Songliao Black Pigs, in 112bp there is a fragment about 289bp insertion mutagenesis; verified NR6A1 gene fragment Nci I restriction sites in Duroc clear effect of three hybrid groups, NciI enzyme fragment of NR6A1 gene restriction sites AA in Songliao Black Pig in group AG, three GG genotypes, and the restriction sites in the Hardy-Weinberg equilibrium in Songliao Black pig population, genetic polymorphism in moderate polymorphism; fragment of VRTN gene of ins/ins, ins/- and - three base Because in the three yuan of Duroc hybrid population and Songliao Black pig population exists, the insertion mutation in Duroc hybrid population and three yuan in Songliao Black pig population were in Hardy-Weinberg equilibrium, genetic polymorphism in moderate polymorphism; NR6A1 gene 184AG locus of Songliao Black Pig individual rib number phenotypic variation phenomenon does not exist Association of VRTN gene 112bp locus mutation and insertion of Duroc Hybrid Pigs and three yuan songliaohei pig rib number of phenotypic variation exists certain relevance, so the insertion mutation is likely to be in the Songliao Black Pig and improve product in breeding the ideal molecular markers of multi rib lines.

【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S828

【共引文献】