日粮中添加大豆抗原蛋白对断奶仔猪血清中IgG和IgE水平以及肠道ZO-1表达的影响

发布时间：2018-03-21 10:45

本文选题：β-伴大豆球蛋白　切入点：大豆球蛋白　出处：《中国兽医科学》2017年03期 　论文类型：期刊论文

【摘要】：为了评价大豆抗原蛋白对断奶仔猪有无致敏作用及对肠紧密连接蛋白ZO-1表达的影响,选取70头24日龄断奶仔猪,随机将其分为7组,A组饲喂基础日粮,B组、C组、D组的饲粮中分别按仔猪初始体重添加500、2 000、5 000 mg/kg的β-伴大豆球蛋白,E组、F组、G组的饲粮中按仔猪初始体重分别添加500、2 000、5 000 mg/kg的大豆球蛋白,通过ELISA检测仔猪24及30日龄时血清中IgG及IgE的质量浓度,并于仔猪30日龄时,每组选取5头仔猪放血致死,采集小肠组织,通过免疫组化检测小肠紧密连接蛋白ZO-1的表达情况,并用实时荧光定量PCR测定ZO-1 m RNA的相对表达量。统计学分析结果表明,在仔猪日粮中添加大豆抗原蛋白能刺激断奶仔猪血清IgG和IgE的质量浓度升高(P0.01),小肠ZO-1蛋白表达和ZO-1 m RNA的相对表达量降低,且大豆抗原蛋白的添加剂量越多,ZO-1表达越低。上述结果表明,β-伴大豆球蛋白和大豆球蛋白能引起仔猪肠道过敏反应,损伤肠道紧密连接;β-伴大豆球蛋白的致敏作用大于大豆球蛋白。
[Abstract]:In order to evaluate the sensitizing effect of soybean antigen protein on weaning piglets and the effect of soybean antigen protein on ZO-1 expression of intestinal tight junction protein, 70 24 day weaned piglets were selected. They were randomly divided into 7 groups: group A, fed basal diet, group B, group C, group C, group D, diet supplemented with 5002 000 ~ 5 000 mg/kg of 尾 -concomitant soybean globulin E group according to the initial body weight of piglets, respectively, the diets of group G were added according to the initial body weight of piglets. Soybean globulin from 500,000,000g / 5 000 mg/kg, The concentration of IgG and IgE in serum of piglets at 24 and 30 days of age was detected by ELISA. At 30 days of age, 5 piglets in each group were selected to bleed to death, and small intestine tissues were collected. The expression of ZO-1 was detected by immunohistochemistry. The relative expression of ZO-1 m RNA was measured by real-time fluorescence quantitative PCR. The addition of soybean antigen protein to the diet of piglets could stimulate the increase of serum IgG and IgE concentration in weaned piglets, while the expression of ZO-1 protein in small intestine and the relative expression of ZO-1 m RNA in small intestine decreased. The more the additive amount of soybean antigen protein, the lower the expression of ZO-1. The above results showed that 尾 -concomitant soybean globulin and soybean globulin could induce intestinal allergic reaction in piglets. The sensitizing effect of 尾-concomitant soybean globulin was greater than that of soybean globulin.
【作者单位】：安徽农业大学动物科技学院;
【基金】：科技部科技富民强县专项行动计划项目(国科发农[2012]745号)
【分类号】：S828.5

【相似文献】