1～21日龄黄羽肉鸡钠、氯的需要量

发布时间：2018-03-21 14:50

本文选题：黄羽肉鸡　切入点：钠　出处：《动物营养学报》2017年03期 　论文类型：期刊论文

【摘要】：本试验旨在通过研究饲粮不同钠、氯水平对黄羽肉鸡生长性能、排泄物含水率、血清指标、肠道钠-葡萄糖共转运载体1(SGLT1)和钠/氢交换载体2(NHE2)mRNA表达量等的影响,探讨1~21日龄阶段黄羽肉鸡钠和氯的需要量及其作用机理。试验选用1日龄健康、发育良好的快大型岭南黄羽肉公鸡共1 200只,根据体重随机分成5个组,每组6个重复,每个重复40只鸡。对照组采用玉米-豆粕型基础饲粮(无额外钠、氯添加),其余各组都以钠、氯含量比1∶1设梯度,通过食盐与碳酸氢钠等量取代部分沸石粉使饲粮钠与氯含量均分别为0.1%、0.2%、0.3%和0.4%,试验期间试鸡自由采食和饮水。试验结果显示,饲粮补充钠、氯显著提高了平均日增重、平均日采食量、平均日饮水量和排泄物含水率(P0.05),显著降低了料重比和死亡率(P0.05),其中平均日增重随饲粮钠、氯水平的提高呈二次折线升高(R2=0.966,P=0.009),排泄物含水率随饲粮钠、氯水平的提高呈先升高后降低的二次曲线变化(R2=0.954,P=0.046);饲粮补充钠、氯显著提高了血清渗透压(P0.05),显著降低了血清尿酸、葡萄糖、总胆固醇和甘油三酯含量(P0.05),对血清胆囊收缩素、胃饥饿素和生长激素含量无显著影响(P0.05);饲粮钠、氯水平均为0.2%、0.4%组十二指肠NHE2 mRNA表达量和钠-钾ATP(Na+-K+-ATP)酶活性显著低于对照组(P0.05);饲粮钠、氯水平为0.2%组回肠SGLT1 mRNA表达量显著高于对照组和0.4%组(P0.05)。结果提示,本试验条件下,以生长性能为评定指标,根据方差分析和多重比较得到,1~21日龄阶段快大型黄羽肉鸡饲粮适宜钠、氯水平均为0.2%;通过非线性回归模型估测得到,饲粮适宜钠、氯水平均为0.14%。
[Abstract]:The purpose of this study was to study the effects of different dietary sodium and chlorine levels on growth performance, fecal moisture content, serum index, intestinal sodium glucose co-transport vector 1SGLT1) and sodium / hydrogen exchange vector 2NHE2 mRNA expression in yellow feather broilers. The requirement of sodium and chlorine and the mechanism of action of sodium and chlorine in 1-day-old yellow feather broiler were studied. 1 200 fast and large Lingnan yellow feather roosters, healthy and well developed, were randomly divided into 5 groups according to their body weight, 6 replicates in each group, and 1 200 of them were randomly divided into 5 groups according to their body weight. The control group was fed a corn-soybean meal diet (no extra sodium, chlorine added to the diet), and the rest groups were graded with sodium and chlorine content ratio of 1: 1. The contents of sodium and chlorine in diet were 0.1% and 0.23%, respectively, by replacing part of boiling powder with salt and sodium bicarbonate, respectively. During the trial period, the chickens were fed freely and drinking water. The results showed that supplementation of sodium and chlorine in diet significantly increased the average daily gain. The average daily intake, average daily drinking water and excreta moisture content were significantly decreased, the ratio of feed to weight and the mortality rate were significantly decreased. The average daily gain increased with dietary sodium, and the increase of chlorine level showed a second fold increase of R2N 0.966g P0. 009N, and the water content of excreta increased with dietary sodium. The level of chlorine increased first and then decreased. The dietary sodium supplementation significantly increased serum osmotic pressure (P0.05N), decreased serum uric acid, glucose, total cholesterol and triglyceride contents (P0.05N), and significantly decreased serum cholecystokinin (Cholecystokinin). There was no significant effect on the contents of hunger and growth hormone in stomach, sodium and chlorine levels in diet were both 0.2%. The expression of NHE2 mRNA and the activity of ATP(Na -K ATPase in duodenum of 0.4% group were significantly lower than those in control group (P 0.05). The expression of SGLT1 mRNA in ileum of 0.2% group was significantly higher than that of control group and 0.4% group. According to the analysis of variance and multiple comparisons, the suitable sodium and chlorine levels were 0.2and 0.14, respectively, in fast yellow feather broilers at 21 days of age, and were estimated by nonlinear regression model.
【作者单位】：广东省农业科学院动物科学研究所畜禽育种国家重点实验室农业部华南动物营养与饲料重点开放实验室广东省动物育种与营养公共实验室广东省畜禽育种与营养研究重点实验室;华中农业大学动物科学技术学院动物医学院;
【基金】：国家肉鸡产业技术体系项目(CARS-42) 国家“十二五”科技支撑计划项目子课题(2014BAD13B02) 广东省科技攻关项目(2013B020306002)
【分类号】：S831.5

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