不同水平肌醇对獭兔皮肤中β-连环蛋白和激素敏感脂酶表达的影响

发布时间：2018-03-21 16:22

本文选题：肌醇　切入点：獭兔　出处：《动物营养学报》2017年08期 　论文类型：期刊论文

【摘要】：本试验旨在研究饲粮中添加不同水平的肌醇对獭兔皮肤中β-连环蛋白(β-catenin)和激素敏感脂酶(HSL)表达的影响。选取(40±1)日龄的同期断奶獭兔120只(公母各占1/2),随机分成4组,每组30只。在4组獭兔的饲粮中分别添加0、25、50、75 mg/kg的肌醇,试验期为3个月。在试验的第30天(2月龄)、第60天(3月龄)和第90天(4月龄),分别取腹部、背中部、臀部皮肤,采用免疫组织化学和蛋白质印迹(Western blot)法对β-catenin和HSL的蛋白质表达和定位进行检测。结果表明:β-catenin在毛囊中广泛表达,在毛根鞘细胞和毛乳头均有棕黄色阳性反应细胞。HSL在毛根鞘细胞,尤其是在内根鞘细胞内,呈现非常明显的棕黑色强阳性表达。饲粮中添加50 mg/kg肌醇可以极显著增加2~4月龄獭兔背中部皮肤毛囊中β-catenin和HSL阳性表达细胞的平均灰度值(P0.01),极显著增加4月龄獭兔背中部、腹部和臀部皮肤毛囊中β-catenin和HSL阳性表达细胞的平均灰度值(P0.01)。结果提示,肌醇能够通过上调4月龄獭兔毛囊中β-catenin和HSL的表达来促进毛囊的发育,在本试验中,饲粮肌醇水平达到50 mg/kg时效果最佳。
[Abstract]:The purpose of this study was to study the effects of different levels of inositol on the expression of 尾 -catenin (尾 -catenin) and glucocorticoid sensitive lipase (HSL) in the skin of Rex rabbits. 120 rex rabbits (male and female) were randomly divided into 4 groups. 30 rabbits in each group were fed with inositol 0255075 mg/kg in the diet of 4 groups for 3 months. On the 30th day of the trial, the rats were 2 months old, 60 days old and 3 months old) and 90 days old respectively. The skin of abdomen, middle back and hip were taken, respectively. The protein expression and localization of 尾 -catenin and HSL were detected by immunohistochemistry and Western blot.The results showed that 尾 -catenin was widely expressed in hair follicles. In both hair root sheath cells and dermal papilla, there were brown positive reaction cells. HSL was found in hair root sheath cells, especially in inner root sheath cells. The positive expression of 尾 -catenin and HSL positive cells in the hair follicles of the middle part of the dorsal skin of rabbits was significantly increased by adding 50 mg/kg inositol to the diet, and the average gray value of 尾 -catenin and HSL positive cells in the hair follicles of the middle part of the dorsal skin of the rabbits was significantly increased (P 0.01), and the middle part of the back of the rex rabbits at the age of 4 months was significantly increased. The average gray value of 尾 -catenin and HSL positive cells in the skin follicles of abdomen and buttocks was P 0.01. The results suggested that inositol could promote the development of hair follicles by up-regulating the expression of 尾 -catenin and HSL in the follicles of 4 month old Rex rabbits. When dietary inositol level reached 50 mg/kg, the effect was the best.
【作者单位】：河北农业大学动物医学院;河北农业大学动物科技学院;
【基金】：国家兔产业技术体系(CARS-44-B-3) 吉林海资生物技术有限公司横向课题
【分类号】：S829.1

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